PUBLICATION
Involvement of anxiety-like behaviors and brain oxidative stress in the chronic effects of alarm reaction in zebrafish populations
- Authors
- Quadros, V.A., Rosa, L.V., Costa, F.V., Müller, T.E., Stefanello, F.V., Loro, V.L., Rosemberg, D.B.
- ID
- ZDB-PUB-190617-2
- Date
- 2019
- Source
- Neurochemistry international 129: 104488 (Journal)
- Registered Authors
- Keywords
- Aversive chemical cue, Conspecific alarm substance, Leopard, Light/dark test, Novel tank test, Wild type
- MeSH Terms
-
- Animals
- Anxiety/chemically induced
- Anxiety/genetics
- Anxiety/physiopathology*
- Avoidance Learning/drug effects
- Avoidance Learning/physiology*
- Brain/metabolism
- Brain/physiopathology*
- Catalase/analysis
- Exploratory Behavior/drug effects
- Exploratory Behavior/physiology*
- Fear/drug effects
- Fear/physiology*
- Female
- Freezing Reaction, Cataleptic/drug effects
- Freezing Reaction, Cataleptic/physiology*
- Glutathione Transferase/analysis
- Lipid Peroxidation/drug effects
- Male
- Nuclear Proteins/deficiency
- Nuclear Proteins/genetics
- Nuclear Proteins/physiology
- Oxidative Stress*/drug effects
- Pheromones/pharmacology
- Protein Carbonylation/drug effects
- Sulfhydryl Compounds/analysis
- Superoxide Dismutase/analysis
- Tissue Extracts/pharmacology
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/analysis
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology
- PubMed
- 31202882 Full text @ Neurochem. Int.
Citation
Quadros, V.A., Rosa, L.V., Costa, F.V., Müller, T.E., Stefanello, F.V., Loro, V.L., Rosemberg, D.B. (2019) Involvement of anxiety-like behaviors and brain oxidative stress in the chronic effects of alarm reaction in zebrafish populations. Neurochemistry international. 129:104488.
Abstract
Aversive conditions elicit anxiety responses that prepare the organism to an eventual threat. Nonetheless, prolonged anxiety is a pathological condition associated with various neuropsychiatric disorders. Here, we evaluated whether the conspecific alarm substance (CAS), a chemical cue that elicits aversion, influences anxiety-like behaviors and modulates brain oxidative stress-related parameters in wild-type (WT) and leopard (leo) zebrafish following a repeated exposure protocol. CAS exposure was performed for 5 min, once daily for 7 consecutive days. In the 8th day, animals were tested in the light/dark and novel tank tests and their brains were further dissected for biochemical analyses. CAS chronically induced anxiogenic-like states in WT and leo populations when their behaviors were analyzed in the light/dark and novel tank tests. CAS also increased catalase (CAT) and glutathione S-transferase (GST) activities, as well as non-protein thiol (NPSH) content in WT and leo, but only leo had increased thiobarbituric reactive substance (TBARS) levels in the brain. At baseline conditions, leo was more 'anxious' when compared to WT, displaying lower CAT activity and carbonylated protein (CP) levels. Overall, CAS chronically triggers anxiety-like behavior in zebrafish populations, which may be associated with changes in oxidative stress-related parameters. Furthermore, the use of different zebrafish populations may serve as an interesting tool in future research to investigate the neurobehavioral bases of neuropsychiatric disorders in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping