PUBLICATION
Nanoliposomes encapsulating immunostimulants modulate the innate immune system and elicit protection in zebrafish larvae
- Authors
- Ji, J., Merino, S., Magaña, J.T., Roher, N.
- ID
- ZDB-PUB-190614-14
- Date
- 2019
- Source
- Fish & shellfish immunology 92: 421-429 (Journal)
- Registered Authors
- Keywords
- Aeromonas hydrophila, Immune response, Nanoliposome, Zebrafish larvae
- MeSH Terms
-
- Adjuvants, Immunologic/administration & dosage
- Adjuvants, Immunologic/pharmacology*
- Aeromonas hydrophila/physiology
- Animals
- Escherichia coli/chemistry
- Fish Diseases/immunology*
- Gram-Negative Bacterial Infections/immunology
- Gram-Negative Bacterial Infections/veterinary
- Immunity, Innate/drug effects*
- Lipopolysaccharides/pharmacology
- Liposomes/administration & dosage
- Liposomes/immunology
- Liposomes/pharmacology*
- Nanoparticles/administration & dosage
- Poly I-C/pharmacology
- Zebrafish/growth & development
- Zebrafish/immunology*
- PubMed
- 31195115 Full text @ Fish Shellfish Immunol.
Citation
Ji, J., Merino, S., Magaña, J.T., Roher, N. (2019) Nanoliposomes encapsulating immunostimulants modulate the innate immune system and elicit protection in zebrafish larvae. Fish & shellfish immunology. 92:421-429.
Abstract
Here we present immunostimulant-loaded nanoliposomes (NLc) as a strategy to protect zebrafish larvae against bacterial infection. The NLc encapsulate crude lipopolysaccharide (LPS) from E. coli and polyinosinic:polycytidylic acid (Poly I:C), a synthetic analogue of viral dsRNA. Fluorescently-labeled NLc were ingested by zebrafish larvae 4 days post fertilization, when administrated by bath immersion, and accumulated in the intestine. RT-qPCR analysis showed the expression of innate immune related genes (tnfα, il1β, nos2a, irf1a and ptgs2a) was significantly upregulated at 48 h post NLc treatment. A zebrafish larvae infection model for Aeromonas hydrophila was set up by bath immersion, achieving bacterial-dose-dependent significant differences in survival at day 5 post infection in both injured and non-injured larvae. Using this model, NLc protected non-injured zebrafish larvae against an A. hydrophila lethal infection. In contrast, neither the empty nanoliposomes nor the mixture of immunostimulants could protect larvae against lethal challenges. Our results demonstrate that nanoliposomes could be further developed as an efficient carrier, widening the scope for delivery of other immunostimulants in aquaculture.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping