PUBLICATION
A novel conserved enhancer at zebrafish zic3 and zic6 loci drives neural expression
- Authors
- Minhas, R., Paterek, A., Łapiński, M., Bazała, M., Korzh, V., Winata, C.L.
- ID
- ZDB-PUB-190614-12
- Date
- 2019
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 248(9): 837-849 (Journal)
- Registered Authors
- Korzh, Vladimir, Minhas, Rashid, Winata, Cecilia Lanny
- Keywords
- cis-regulation, conserved non-coding elements, development, gene regulation, habenula, transgenic zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Conserved Sequence
- Embryonic Development/genetics
- Enhancer Elements, Genetic*
- Gene Expression Regulation, Developmental*
- Green Fluorescent Proteins/metabolism
- Habenula/embryology
- Habenula/growth & development
- Homeodomain Proteins/genetics*
- Humans
- Nervous System/metabolism*
- Repressor Proteins/genetics*
- Transcription Factors/genetics*
- Zebrafish
- Zebrafish Proteins/genetics*
- PubMed
- 31194899 Full text @ Dev. Dyn.
Citation
Minhas, R., Paterek, A., Łapiński, M., Bazała, M., Korzh, V., Winata, C.L. (2019) A novel conserved enhancer at zebrafish zic3 and zic6 loci drives neural expression. Developmental Dynamics : an official publication of the American Association of Anatomists. 248(9):837-849.
Abstract
Background Identifying enhancers and deciphering their putative roles represent a major step to better understand the mechanism of metazoan gene regulation, development, and the role of regulatory elements in disease. Comparative genomics and transgenic assays have been used with some success to identify critical regions that are involved in regulating the spatiotemporal expression of genes during embryogenesis.
Results We identified two novel tetrapod-teleost conserved non-coding elements within the vicinity of the zic3 and zic6 loci in the zebrafish genome and demonstrated their ability to drive tissue-specific expression in a transgenic zebrafish assay. The syntenic analysis and robust green fluorescent expression in the developing habenula in the stable transgenic line were correlated with known sites of endogenous zic3 and zic6 expression.
Conclusion This transgenic line that expresses green fluorescent protein in the habenula is a valuable resource for studying a specific population of cells in the zebrafish central nervous system. Our observations indicate that a genomic sequence that is conserved between humans and zebrafish acts as an enhancer that likely controls zic3 and zic6 expression. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping