PUBLICATION
Internal Versus External Pressures: Effect of Housing Systems on the Zebrafish Microbiome
- Authors
- Breen, P., Winters, A.D., Nag, D., Ahmad, M.M., Theis, K.R., Withey, J.H.
- ID
- ZDB-PUB-190601-7
- Date
- 2019
- Source
- Zebrafish 16(4): 388-400 (Journal)
- Registered Authors
- Keywords
- intestinal microbiome, microbiome, zebrafish housing
- MeSH Terms
-
- Animals
- Gastrointestinal Microbiome*
- Housing, Animal*
- RNA, Bacterial/analysis
- RNA, Ribosomal, 16S/analysis
- Water Microbiology*
- Zebrafish/microbiology*
- PubMed
- 31145047 Full text @ Zebrafish
Citation
Breen, P., Winters, A.D., Nag, D., Ahmad, M.M., Theis, K.R., Withey, J.H. (2019) Internal Versus External Pressures: Effect of Housing Systems on the Zebrafish Microbiome. Zebrafish. 16(4):388-400.
Abstract
Zebrafish (Danio rerio) are an attractive model organism for scientific studies, including host-microbe interactions. The organism is particularly useful for studying aquatic microbes that can colonize vertebrate hosts, including Vibrio cholerae. Previous studies have established the presence of a core zebrafish intestinal microbiome, and tracked the development of the zebrafish intestinal microbiome from the larval stage to adulthood. An unexplored matter in this host-microbe relationship is the effect of the housing system on the zebrafish intestinal and tank water microbiomes. In this study, we used 16S rRNA gene sequencing to investigate the response of zebrafish intestinal and tank water microbiomes to a change in housing conditions. Zebrafish in the separated fish tanks showed no initial differences in the structures of their intestinal microbial profiles; the same prominent bacteria were present and abundant across tanks. Immediately after the housing switch, the zebrafish intestinal microbial profiles changed in composition and structure. Within 5 days of the housing switch, the intestinal microbiome had stabilized, and changed significantly from the prehousing switch profile. This study demonstrates that although external factors can significantly perturb and alter the zebrafish intestinal microbiome, the microbiome displays a large level of selective resilience whose primary members (namely Vibrio) persist.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping