PUBLICATION
Antiviral efficiency of a coumarin derivative on spring viremia of carp virus in vivo
- Authors
- Liu, G., Wang, C., Wang, H., Zhu, L., Zhang, H., Wang, Y., Pei, C., Liu, L.
- ID
- ZDB-PUB-190517-6
- Date
- 2019
- Source
- Virus research 268: 11-17 (Journal)
- Registered Authors
- Keywords
- Antiviral, Coumarin, Horizontal transmission, Rhabdovirus, Zebrafish
- MeSH Terms
-
- Animals
- Antiviral Agents/chemistry
- Antiviral Agents/therapeutic use*
- Aquaculture
- Coumarins/chemistry
- Coumarins/therapeutic use*
- Fish Diseases/drug therapy*
- Fish Diseases/virology
- Rhabdoviridae/drug effects*
- Rhabdoviridae Infections/drug therapy
- Rhabdoviridae Infections/veterinary*
- Viral Load/drug effects
- Zebrafish
- PubMed
- 31095989 Full text @ Virus Res.
Citation
Liu, G., Wang, C., Wang, H., Zhu, L., Zhang, H., Wang, Y., Pei, C., Liu, L. (2019) Antiviral efficiency of a coumarin derivative on spring viremia of carp virus in vivo. Virus research. 268:11-17.
Abstract
Spring viraemia of carp (SVC) in aquaculture is challenging because there are few preventative measures and/or treatments. The previous study demonstrated that an antiviral coumarin derivative, 7-(4-(4-methyl-imidazole))-coumarin (C2), inhibits spring viremia of carp virus (SVCV) infection by targeting Nrf2-ARE signaling pathway in fish cells. Thus, we hypothesized whether C2 may be used as a potential therapeutic agent for controlling SVCV infection in aquaculture. In this study, SVCV infectivity was significantly inhibited in vitro in a dose-dependent manner by preincubation with C2. C2 was verified against SVCV in zebrafish, in which the mortality and viral titer in fish body were decreased. Like other coumarins, C2 was stable with a prolonged inhibitory half-life (3.5 days) at 15 °C in the early stage of SVCV infection. The results show that horizontal transmission of SVCV was reduced by C2 in a static cohabitation challenge model, especially for recipient fish in injection treatment, which suggested that C2 may be suitable as a possible therapeutic agent for SVCV in aquaculture. Overall, this study provides the new insight that a small molecule antiviral drug can be used to control rhabdovirus infection in fish aquacultures.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping