PUBLICATION
Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish
- Authors
- Li, H., Zhang, W., Zhang, H., Xie, Y., Sun, C., Di, C., Si, J., Gan, L., Yan, J.
- ID
- ZDB-PUB-190516-13
- Date
- 2019
- Source
- Journal of Cellular Physiology 234(12): 22439-22449 (Journal)
- Registered Authors
- Li, Hongyan
- Keywords
- carbon ion radiation, mitochondria, spermatogenic cells, zebrafish
- MeSH Terms
-
- Animals
- Apoptosis/radiation effects*
- Carbon*
- Cell Proliferation/radiation effects
- Gene Expression Regulation/radiation effects
- Heavy Ion Radiotherapy/adverse effects*
- Heavy Ions*
- Male
- Mitochondria/metabolism
- Mitochondria/radiation effects
- Mitochondrial Proteins/genetics
- Mitochondrial Proteins/metabolism
- Proteomics
- Spermatogonia/drug effects*
- Testis/cytology*
- Testis/radiation effects
- X-Rays
- Zebrafish
- PubMed
- 31087336 Full text @ J. Cell. Physiol.
Citation
Li, H., Zhang, W., Zhang, H., Xie, Y., Sun, C., Di, C., Si, J., Gan, L., Yan, J. (2019) Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish. Journal of Cellular Physiology. 234(12):22439-22449.
Abstract
The mitochondrial proteins involved in spermatogenic cells apoptosis in zebrafish after carbon ion radiation (CIR) were screened. The relative biological effectiveness (RBE) of CIR in zebrafish testes was investigated. Apoptosis of testicular cells was measured within 24 hr following 1 and 4 Gy CIR. Immunoblotting was used to assess the levels of mitochondrial apoptotic proteins in testes, and proliferative and apoptotic spermatogenic cells were detected by immunofluorescence after CIR. Label-free quantitative (LFQ) and parallel reaction monitoring-based target proteomics (PRM) were combined to screen and validate differential mitochondrial proteins in testes between 4 Gy and control groups at 24 hr after CIR. The RBE of CIR in zebrafish testes was 1.48 ± 0.04, and induction of apoptosis by CIR was higher than that of X-rays in testicular cells. Mitochondrial apoptotic pathways play a crucial role in spermatogenic cells apoptosis after CIR, with 60 differential mitochondrial proteins identified. Among 20 target proteins, 12 were significantly upregulated, 2 were significantly downregulated in the 4 Gy CIR group. The results of PRM were consistent with label-free analysis. This is the first study to screen the differential mitochondrial proteins and provide useful information to understand the underlying mechanisms of spermatogenic cell apoptosis in zebrafish following CIR.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping