PUBLICATION
The PFOS disturbed immunomodulatory functions via nuclear Factor-κB signaling in liver of zebrafish (Danio rerio)
- Authors
- Guo, J., Wu, P., Cao, J., Luo, Y., Chen, J., Wang, G., Guo, W., Wang, T., He, X.
- ID
- ZDB-PUB-190515-1
- Date
- 2019
- Source
- Fish & shellfish immunology 91: 87-98 (Journal)
- Registered Authors
- Keywords
- Immunomodulatory functions, Liver, NF-κB signaling pathway, PFOS, Zebrafish
- MeSH Terms
-
- Alkanesulfonic Acids/toxicity*
- Animals
- Dose-Response Relationship, Drug
- Fish Proteins/genetics
- Fish Proteins/immunology*
- Fluorocarbons/toxicity*
- Liver/drug effects*
- Liver/metabolism
- Male
- NF-kappa B/genetics
- NF-kappa B/immunology*
- Random Allocation
- Reactive Oxygen Species/metabolism
- Signal Transduction/drug effects
- Thiocarbamates/toxicity
- Water Pollutants, Chemical/toxicity*
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/immunology
- Zebrafish/physiology*
- PubMed
- 31082517 Full text @ Fish Shellfish Immunol.
Citation
Guo, J., Wu, P., Cao, J., Luo, Y., Chen, J., Wang, G., Guo, W., Wang, T., He, X. (2019) The PFOS disturbed immunomodulatory functions via nuclear Factor-κB signaling in liver of zebrafish (Danio rerio). Fish & shellfish immunology. 91:87-98.
Abstract
Excessive perfluorooctane sulfonate (PFOS) in natural water ecosystem has the potential to detrimentally affect immune system, but little is known of such effects or underlying mechanisms in fish. In the present study, we evaluated the effects of PFOS on growth performance, organizational microstructure, activities of immune related-enzymes and expressions of immune related-genes in male zebrafish (Danio rerio) exposed to different concentrations of 0, 0.02, 0.04 and 0.08 mg/L of PFOS for 7, 14, and 21 days to investigate the effects of PFOS on immune system and the potential toxic mechanisms caused by PFOS. The results indicated that PFOS accumulated in livers after exposure, and remarkably elevations were found in three exposure groups compared with the control group at three stages. The growth of the adult zebrafish in the experiments was significantly inhibited, the liver microstructures of live were serious damaged. The ROS levels were remarkably increased. The activities of ACP, AKP, and lysozyme were obviously decreased, while the activities of MPO and NF-κB were significantly increased. The expressions of immune-related mRNA were significantly affected. After co-treatment with PFOS and PDTC, the growth inhibition, the morphological damage, the ROS induction, and the expressions of immune-related mRNA were reversed. Taken together, the results indicated that PFOS can significantly inhibit the growth, disturb the immune system by changing the normal structure of liver, the activities of immune-related enzymes, and a series of gene transcriptions involved in immune regulation in liver of male zebrafish. PFOS-induced pro-inflammatory effect of hepatocytes was observed, and the involvement of NF-κB signaling pathway was participated in its action mechanism. These findings provide further evidence that PFOS interferes with the immune regulation of liver under in vivo conditions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping