PUBLICATION
Circulating factors cause proteinuria in parabiotic zebrafish
- Authors
- Müller-Deile, J., Schenk, H., Schroder, P., Schulze, K., Bolaños-Palmieri, P., Siegerist, F., Endlich, N., Haller, H., Schiffer, M.
- ID
- ZDB-PUB-190512-2
- Date
- 2019
- Source
- Kidney International 96(2): 342-349 (Journal)
- Registered Authors
- Keywords
- nephrotic syndrome, podocyte, proteinuria
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Extracellular Matrix Proteins/genetics*
- Gene Expression Regulation
- Gene Knockdown Techniques
- Glomerulosclerosis, Focal Segmental/blood*
- Glomerulosclerosis, Focal Segmental/genetics
- Glomerulosclerosis, Focal Segmental/pathology
- Humans
- Microscopy, Electron, Transmission
- Morpholinos/genetics
- Parabiosis
- Podocytes/pathology*
- Podocytes/ultrastructure
- Proteinuria/blood*
- Proteinuria/genetics
- Proteinuria/pathology
- Zebrafish
- Zebrafish Proteins/blood
- Zebrafish Proteins/genetics*
- PubMed
- 31076096 Full text @ Kidney Int.
Citation
Müller-Deile, J., Schenk, H., Schroder, P., Schulze, K., Bolaños-Palmieri, P., Siegerist, F., Endlich, N., Haller, H., Schiffer, M. (2019) Circulating factors cause proteinuria in parabiotic zebrafish. Kidney International. 96(2):342-349.
Abstract
Proteinuria can be induced by impairment of any component of the glomerular filtration barrier. To determine the role of circulating permeability factors on glomerular damage, we developed a parabiosis-based zebrafish model to generate a common circulation between zebrafish larvae. A morpholino-mediated knockdown of a podocyte specific gene (nephronectin) was induced in one zebrafish larva which was then fused to an un-manipulated fish. Notably, proteinuria and glomerular damage were present in the manipulated fish and in the parabiotically-fused partner. Thus, circulating permeability factors may be induced by proteinuria even when an induced podocyte gene dysregulation is the initiating cause.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping