PUBLICATION
Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology
- Authors
- Warner, B.K., Alder, J.K., Suli, A.
- ID
- ZDB-PUB-190510-10
- Date
- 2019
- Source
- Methods in molecular biology (Clifton, N.J.) 1965: 235-250 (Chapter)
- Registered Authors
- Suli, Arminda
- Keywords
- CRISPR, Cas9, Clustered regularly interspaced short palindromic repeats, Genomic editing, Morpholino, Zebrafish
- MeSH Terms
-
- Animals
- CRISPR-Cas Systems
- Embryonic Development/drug effects
- Gene Editing/methods*
- Gene-Environment Interaction
- Teratogens/toxicity
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 31069679 Full text @ Meth. Mol. Biol.
Citation
Warner, B.K., Alder, J.K., Suli, A. (2019) Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology. Methods in molecular biology (Clifton, N.J.). 1965:235-250.
Abstract
Environment-gene interactions have a powerful impact on embryo development. The ability to precisely edit the genome makes it possible to address questions concerning the specific roles that genes or variants play in modulating the response to environmental challenges. In this chapter, we provide a simplified protocol using CRISPR-Cas9 ribonucleoproteins for genome editing in the zebrafish model organism. The genetic manipulation can then be coupled with chemical screens to identify and understand the mechanism behind toxicants or compounds that modulate development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping