ZFIN ID: ZDB-PUB-190507-33
Brain transcriptome analysis of a familial Alzheimer's disease-like mutation in the zebrafish presenilin 1 gene implies effects on energy production
Newman, M., Hin, N., Pederson, S., Lardelli, M.
Date: 2019
Source: Molecular brain   12: 43 (Journal)
Registered Authors: Lardelli, Michael, Newman, Morgan
Keywords: ATP synthesis, Alzheimer’s disease, Brain, Genome editing, Mitochondria, Mutation, Presenilin 1, Transcriptome, Vacuolar acidification, Zebrafish
Microarrays: GEO:GSE126096
MeSH Terms:
  • Alzheimer Disease/genetics*
  • Animals
  • Brain/metabolism*
  • Energy Metabolism/genetics*
  • Gene Expression Profiling*
  • Gene Expression Regulation
  • Gene Ontology
  • Heterozygote
  • Humans
  • Mutation/genetics*
  • Presenilin-1/genetics*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed: 31053140 Full text @ Mol. Brain
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ABSTRACT
To prevent or ameliorate Alzheimer's disease (AD) we must understand its molecular basis. AD develops over decades but detailed molecular analysis of AD brains is limited to postmortem tissue where the stresses initiating the disease may be obscured by compensatory responses and neurodegenerative processes. Rare, dominant mutations in a small number of genes, but particularly the gene PRESENILIN 1 (PSEN1), drive early onset of familial AD (EOfAD). Numerous transgenic models of AD have been constructed in mouse and other organisms, but transcriptomic analysis of these models has raised serious doubts regarding their representation of the disease state. Since we lack clarity regarding the molecular mechanism(s) underlying AD, we posit that the most valid approach is to model the human EOfAD genetic state as closely as possible. Therefore, we sought to analyse brains from zebrafish heterozygous for a single, EOfAD-like mutation in their PSEN1-orthologous gene, psen1. We previously introduced an EOfAD-like mutation (Q96_K97del) into the endogenous psen1 gene of zebrafish. Here, we analysed transcriptomes of young adult (6-month-old) entire brains from a family of heterozygous mutant and wild type sibling fish. Gene ontology (GO) analysis implies effects on mitochondria, particularly ATP synthesis, and on ATP-dependent processes including vacuolar acidification.
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