PUBLICATION
Characterization of Intracellular Peptides from Zebrafish (Danio rerio) Brain
- Authors
- Teixeira, C.M.M., Correa, C.N., Iwai, L.K., Ferro, E.S., Castro, L.M.
- ID
- ZDB-PUB-190425-17
- Date
- 2019
- Source
- Zebrafish 16(3): 240-251 (Journal)
- Registered Authors
- Keywords
- intracellular peptides, mass spectrometry, nervous system, peptidome
- MeSH Terms
-
- Animals
- Brain/metabolism
- Fish Proteins/genetics*
- Fish Proteins/metabolism
- Intracellular Signaling Peptides and Proteins/genetics*
- Intracellular Signaling Peptides and Proteins/metabolism
- Proteome/genetics*
- Proteome/metabolism
- Sequence Analysis, RNA
- Zebrafish/genetics*
- PubMed
- 31017557 Full text @ Zebrafish
Citation
Teixeira, C.M.M., Correa, C.N., Iwai, L.K., Ferro, E.S., Castro, L.M. (2019) Characterization of Intracellular Peptides from Zebrafish (Danio rerio) Brain. Zebrafish. 16(3):240-251.
Abstract
Peptides represent a large class of cell signaling molecules, and they are mainly produced by the classical secretory pathway or during protein degradation. The peptide profile of Danio rerio (zebrafish) shows a lack of information when compared with other consolidated animal models. The aim of this work was to characterize the peptide profile of zebrafish brain by using triplex reductive methylation of amines labeling and liquid chromatography coupled to electron spray mass spectrometry. A total of 411 peptide fragments were detected and 125 peptide sequences could be solved. Further analysis suggested that most of the peptides were fragments of intracellular cytosolic and mitochondrial proteins, and that 60% of the precursor proteins were cleaved at either their N- or C-terminal. The most common residue in the P1 position was leucine whereas other common residues were lysine, alanine, arginine, and phenylalanine. Rare cleavage sites at P1 position were histidine, glutamic acid, and isoleucine. The peptide profile of zebrafish brain has similarities with results previously described in mice brain peptidome studies. Thus, this study represents an important basis for the molecular understanding of zebrafish and its use as a model for human diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping