PUBLICATION
Characterization and kinetic study of the brain and muscle acetylcholinesterase from Danio rerio
- Authors
- Marinho, C.S., Matias, M.V.F., Brandão, I.G.F., Santos, E.L., Machado, S.S., Zanta, C.L.P.S.
- ID
- ZDB-PUB-190416-6
- Date
- 2019
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 222: 11-18 (Journal)
- Registered Authors
- Keywords
- Acetylcholinesterase, Danio rerio, Inhibitors, Kinetics
- MeSH Terms
-
- Acetylcholinesterase/metabolism*
- Animals
- Brain/enzymology*
- Cholinesterase Inhibitors/administration & dosage
- Cholinesterase Inhibitors/pharmacology
- Dose-Response Relationship, Drug
- Hydrogen-Ion Concentration
- Muscle, Skeletal/enzymology*
- Temperature
- Zebrafish/metabolism*
- PubMed
- 30981910 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Marinho, C.S., Matias, M.V.F., Brandão, I.G.F., Santos, E.L., Machado, S.S., Zanta, C.L.P.S. (2019) Characterization and kinetic study of the brain and muscle acetylcholinesterase from Danio rerio. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 222:11-18.
Abstract
Acetylcholinesterase (AChE) plays an important role in the therapy of Alzheimer's disease and in the detection of pesticides such as organophosphates which are also widely used in chemical warfare. The aim of this study is the physicochemical and kinetic characterization of brain and muscle ChE from Danio rerio (Zebrafish). Optimal activity was found for brain ChE at alkaline pH 9.0 at 30 °C, and for muscle ChE at alkaline pH 8.5 at temperatures between 20 °C and 35 °C. The apparent kinetic constants, Kmapp and Vmaxapp, for brain ChE were determined as 0.191 ± 0.024 mM and 0.566 ± 0.028 U/mg protein, and for muscle ChE as 0.230 ± 0.030 mM and 0.677 ± 0.039 U/mg protein. Both brain and muscle ChE showed inhibition at high substrate concentrations. Brain and muscle ChE showed IC50 values for physostigmine of 0.61 μM and 0.37 μM, respectively. The ChE activity in brain was significantly inhibited by BW254c51 in all concentrations tested, but not by Iso-OMPA, while muscle ChE presented a moderate decrease (13 to 29%) in the activity values, indicating that BuChE is present.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping