PUBLICATION
Neural Crest Methodologies in Zebrafish and Medaka
- Authors
- Petratou, K., Camargo-Sosa, K., Al Jabri, R., Nagao, Y., Kelsh, R.N.
- ID
- ZDB-PUB-190413-5
- Date
- 2019
- Source
- Methods in molecular biology (Clifton, N.J.) 1976: 167-184 (Chapter)
- Registered Authors
- Kelsh, Robert, Nagao, Yusuke
- Keywords
- Immunofluorescence, In situ hybridization, Medaka, Neural crest, Pigment cells, Protein detection RNAscope, Transcript detection, Zebrafish
- MeSH Terms
-
- Animals
- Cell Movement/physiology
- Gene Expression Regulation, Developmental/physiology
- In Situ Hybridization
- Neural Crest/cytology*
- Oryzias
- Zebrafish
- PubMed
- 30977073 Full text @ Meth. Mol. Biol.
Citation
Petratou, K., Camargo-Sosa, K., Al Jabri, R., Nagao, Y., Kelsh, R.N. (2019) Neural Crest Methodologies in Zebrafish and Medaka. Methods in molecular biology (Clifton, N.J.). 1976:167-184.
Abstract
Neural crest cells are highly multipotent and strongly migratory cells and generate adult neural crest stem cells with varied roles in cellular homeostasis and regeneration. The optical transparency and ready accessibility of fish embryos make them particularly well-suited to high-resolution analysis of neural crest development. However, the dispersive nature of these cells adds to the challenge of their study, requiring that they be identified using marker expression. We describe key protocols for the analysis of neural crest marker expression in zebrafish and medaka, including whole-mount in situ hybridization to detect mRNA using conventional chromogenic substrates and the more recent RNAscope which gives readily multiplexed fluorescent detection and immunofluorescent detection of antigens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping