PUBLICATION
Peptidomic analysis of zebrafish embryos exposed to polychlorinated biphenyls and their impact on eye development
- Authors
- Jiang, Y., Zhang, S., Zhang, X., Li, N., Zhang, Q., Guo, X., Chi, X., Tong, M.
- ID
- ZDB-PUB-190322-13
- Date
- 2019
- Source
- Ecotoxicology and environmental safety 175: 164-172 (Journal)
- Registered Authors
- Keywords
- LC-MS/MS, Ocular development, Peptide, Polychlorinated biphenyls, Zebrafish
- MeSH Terms
-
- Animals
- Biomarkers/metabolism
- Child
- Chromatography, Liquid
- Environmental Exposure*
- Environmental Pollutants/adverse effects*
- Eye/drug effects*
- Eye/growth & development
- Humans
- Peptides/metabolism*
- Polychlorinated Biphenyls/adverse effects*
- Tandem Mass Spectrometry
- Zebrafish
- PubMed
- 30897415 Full text @ Ecotoxicol. Environ. Saf.
Citation
Jiang, Y., Zhang, S., Zhang, X., Li, N., Zhang, Q., Guo, X., Chi, X., Tong, M. (2019) Peptidomic analysis of zebrafish embryos exposed to polychlorinated biphenyls and their impact on eye development. Ecotoxicology and environmental safety. 175:164-172.
Abstract
Polychlorinated biphenyls (PCBs), a class of persistent organic pollutant, are closely related to abnormal eye development in children. However, little is known regarding the role of peptides in the development of PCB-induced ocular dysplasia. To characterize the nature of PCB exposure on peptides involved in the development of the ocular system, we used liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) to detect differential expression of peptides between normal and PCB-exposed zebrafish embryos. A total of 7900 peptides were analyzed, 90 of which were differentially expressed, with 29 being up-regulated and 61 down-regulated. These peptides were investigated using ingenuity pathway analysis (IPA) and gene ontology (GO) analysis to explore their role in eye development. This study identified 18 peptides associated with the development of the optic nerve and ocular system in the PCB-exposure group, as well as 10 peptides that are located in the functional domain of their precursor proteins. These peptides provide potential biomarkers for the treatment of ocular dysplasia caused by PCBs and may help us understand the mechanism of abnormal eye development caused by organic pollutants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping