PUBLICATION
In vivo monitoring and high-resolution characterizing of the prednisolone-induced osteoporotic process on adult zebrafish by optical coherence tomography
- Authors
- Lin, Y., Xiang, X., Chen, T., Gao, C., Fu, H., Wang, L., Deng, L., Zeng, L., Zhang, J.
- ID
- ZDB-PUB-190321-9
- Date
- 2019
- Source
- Biomedical Optics Express 10: 1184-1195 (Journal)
- Registered Authors
- Gao, Chuan, Zhang, Jian
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 30891338 Full text @ Biomed. Opt. Express
Citation
Lin, Y., Xiang, X., Chen, T., Gao, C., Fu, H., Wang, L., Deng, L., Zeng, L., Zhang, J. (2019) In vivo monitoring and high-resolution characterizing of the prednisolone-induced osteoporotic process on adult zebrafish by optical coherence tomography. Biomedical Optics Express. 10:1184-1195.
Abstract
Because of its similar genetic makeup with humans, zebrafish are an available and well-established osteoporosis model in vivo for anti-osteoporosis drug development as well as the drug safety-evaluation process. However, few optical imaging methods could effectively visualize the bone of adult zebrafish due to their limited penetration depth. In this paper, in vivo high-resolution and long-term characterization of a prednisolone-induced osteoporotic zebrafish model was achieved with spectral-domain optical coherence tomography (SD-OCT). The capability of three-dimensional SD-OCT imaging was also demonstrated in this study. With SD-OCT images, we could non-destructively monitor the deforming process of adult zebrafish skull from several directions at any time. There is good correlation and agreement between SD-OCT and histology. Valuable phenomenon such as bone defects could be quantitatively evaluated using the SD-OCT images at different time points during a period of 21 days.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping