PUBLICATION
Met-enkephalin inhibits ROS production through Wnt/β-catenin signaling in the ZF4 cells of zebrafish
- Authors
- Liu, Z., Liu, Y., Gu, Y., Gao, L., Li, A., Liu, D., Kang, C., Pang, Q., Wang, X., Han, Q., Yu, H.
- ID
- ZDB-PUB-190314-3
- Date
- 2019
- Source
- Fish & shellfish immunology 88: 432-440 (Journal)
- Registered Authors
- Keywords
- Met-enkephalin, ROS, Wnt/β-catenin signaling, Zebrafish ZF4 cells
- MeSH Terms
-
- Animals
- CCAAT-Enhancer-Binding Protein-alpha/metabolism
- Catalase/metabolism
- Cell Survival
- Cells, Cultured
- Enkephalin, Methionine/pharmacology*
- Glutathione Peroxidase/metabolism
- Hydrogen Peroxide/metabolism
- Membrane Potential, Mitochondrial
- Neurotransmitter Agents/pharmacology*
- Reactive Oxygen Species/metabolism*
- Superoxide Dismutase/metabolism
- Wnt Signaling Pathway*
- Zebrafish*
- beta Catenin/metabolism
- PubMed
- 30862518 Full text @ Fish Shellfish Immunol.
Citation
Liu, Z., Liu, Y., Gu, Y., Gao, L., Li, A., Liu, D., Kang, C., Pang, Q., Wang, X., Han, Q., Yu, H. (2019) Met-enkephalin inhibits ROS production through Wnt/β-catenin signaling in the ZF4 cells of zebrafish. Fish & shellfish immunology. 88:432-440.
Abstract
Opioid neuropeptides are developed early in the course of a long evolutionary process. As the endogenous messengers of immune system, opioid neuropeptides participate in regulating immune response. In this study, the mechanism that Met-enkephalin (M-ENK) inhibits ROS production through Wnt/β-catenin signaling was investigated in the ZF4 cells of zebrafish. ZF4 cells were exposed to 0, 10, 20, 40, 80, and 160 μM Met-enkephalin (M-ENK) for 24 h, and the cell viability was detected with 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. The cell viability was significantly increased by 10, 20, 40, 80, and 160 μM M-ENK. After ZF4 cells were exposed to 0, 20, 40, and 80 μM M-ENK for 24 h, the mRNA expression of Wnt10b, β-catenin, and CCAAT/enhancer binding protein α (C/EBPα) was significantly increased by 40 and 80 μM M-ENK. However, the mRNA and protein expression of GSK-3β was significantly decreased by 40 and 80 μM M-ENK. The protein expression of β-catenin was significantly induced by 40 and 80 μM M-ENK, while the protein expression of p-β-catenin was significantly decreased by 20, 40, and 80 μM M-ENK. In addition, the mRNA expression of CAT, SOD, and GSH-PX was significantly increased by 20, 40, and 80 μM M-ENK. The levels of H2O2, ·OH, and O2·- were significantly decreased, but the activity of CAT, SOD, and GSH-PX was significantly increased by 40 and 80 μM M-ENK. The fluorescence intensity of reactive oxygen species (ROS) was decreased, and the fluorescence intensity of mitochondrial membrane potential (MMP) was increased with the increase of M-ENK concentration in ZF4 cells. The results showed that M-ENK could induce Wnt/β-catenin signaling, which further inhibited ROS production through the induction of C/EBPα, MMP, and the activities of antioxidant enzymes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping