ZFIN ID: ZDB-PUB-190314-3
Met-enkephalin inhibits ROS production through Wnt/β-catenin signaling in the ZF4 cells of zebrafish
Liu, Z., Liu, Y., Gu, Y., Gao, L., Li, A., Liu, D., Kang, C., Pang, Q., Wang, X., Han, Q., Yu, H.
Date: 2019
Source: Fish & shellfish immunology   88: 432-440 (Journal)
Registered Authors:
Keywords: Met-enkephalin, ROS, Wnt/β-catenin signaling, Zebrafish ZF4 cells
MeSH Terms:
  • Animals
  • CCAAT-Enhancer-Binding Protein-alpha/metabolism
  • Catalase/metabolism
  • Cell Survival
  • Cells, Cultured
  • Enkephalin, Methionine/pharmacology*
  • Glutathione Peroxidase/metabolism
  • Hydrogen Peroxide/metabolism
  • Membrane Potential, Mitochondrial
  • Neurotransmitter Agents/pharmacology*
  • Reactive Oxygen Species/metabolism*
  • Superoxide Dismutase/metabolism
  • Wnt Signaling Pathway*
  • Zebrafish*
  • beta Catenin/metabolism
PubMed: 30862518 Full text @ Fish Shellfish Immunol.
ABSTRACT
Opioid neuropeptides are developed early in the course of a long evolutionary process. As the endogenous messengers of immune system, opioid neuropeptides participate in regulating immune response. In this study, the mechanism that Met-enkephalin (M-ENK) inhibits ROS production through Wnt/β-catenin signaling was investigated in the ZF4 cells of zebrafish. ZF4 cells were exposed to 0, 10, 20, 40, 80, and 160 μM Met-enkephalin (M-ENK) for 24 h, and the cell viability was detected with 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. The cell viability was significantly increased by 10, 20, 40, 80, and 160 μM M-ENK. After ZF4 cells were exposed to 0, 20, 40, and 80 μM M-ENK for 24 h, the mRNA expression of Wnt10b, β-catenin, and CCAAT/enhancer binding protein α (C/EBPα) was significantly increased by 40 and 80 μM M-ENK. However, the mRNA and protein expression of GSK-3β was significantly decreased by 40 and 80 μM M-ENK. The protein expression of β-catenin was significantly induced by 40 and 80 μM M-ENK, while the protein expression of p-β-catenin was significantly decreased by 20, 40, and 80 μM M-ENK. In addition, the mRNA expression of CAT, SOD, and GSH-PX was significantly increased by 20, 40, and 80 μM M-ENK. The levels of H2O2, ·OH, and O2·- were significantly decreased, but the activity of CAT, SOD, and GSH-PX was significantly increased by 40 and 80 μM M-ENK. The fluorescence intensity of reactive oxygen species (ROS) was decreased, and the fluorescence intensity of mitochondrial membrane potential (MMP) was increased with the increase of M-ENK concentration in ZF4 cells. The results showed that M-ENK could induce Wnt/β-catenin signaling, which further inhibited ROS production through the induction of C/EBPα, MMP, and the activities of antioxidant enzymes.
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