PUBLICATION
Imaging neural events in zebrafish larvae with linear structured illumination light sheet fluorescence microscopy
- Authors
- Liu, Y., Dale, S., Ball, R., VanLeuven, A.J., Sornborger, A., Lauderdale, J.D., Kner, P.
- ID
- ZDB-PUB-190312-7
- Date
- 2019
- Source
- Neurophotonics 6: 015009 (Journal)
- Registered Authors
- Ball, Rebecca, Lauderdale, James D.
- Keywords
- light sheet fluorescence microscopy, microscopy, optical sectioning, structured illumination
- MeSH Terms
- none
- PubMed
- 30854407 Full text @ Neurophotonics
Citation
Liu, Y., Dale, S., Ball, R., VanLeuven, A.J., Sornborger, A., Lauderdale, J.D., Kner, P. (2019) Imaging neural events in zebrafish larvae with linear structured illumination light sheet fluorescence microscopy. Neurophotonics. 6:015009.
Abstract
Light sheet fluorescence microscopy (LSFM) is a powerful tool for investigating model organisms including zebrafish. However, due to scattering and refractive index variations within the sample, the resulting image often suffers from low contrast. Structured illumination (SI) has been combined with scanned LSFM to remove out-of-focus and scattered light using square-law detection. Here, we demonstrate that the combination of LSFM with linear reconstruction SI can further increase resolution and contrast in the vertical and axial directions compared to the widely adopted root-mean square reconstruction method while using the same input images. We apply this approach to imaging neural activity in 7-day postfertilization zebrafish larvae. We imaged two-dimensional sections of the zebrafish central nervous system in two colors at an effective frame rate of 7 frames per second.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping