PUBLICATION
Screening drugs for myocardial disease in vivo with zebrafish: an expert update
- Authors
- Zhao, Y., Zhang, K., Sips, P., MacRae, C.A.
- ID
- ZDB-PUB-190308-1
- Date
- 2019
- Source
- Expert opinion on drug discovery 14(4): 343-353 (Review)
- Registered Authors
- MacRae, Calum A.
- Keywords
- Zebrafish, cardiovascular, phenotype, screening
- MeSH Terms
-
- Systems Biology/methods
- Cardiovascular Diseases/drug therapy*
- Cardiovascular Diseases/physiopathology
- Humans
- Cardiovascular Agents/administration & dosage
- Cardiovascular Agents/pharmacology
- Zebrafish
- Drug Discovery/methods*
- Disease Models, Animal
- Cardiomyopathies/drug therapy*
- Cardiomyopathies/physiopathology
- Animals
- Drug Evaluation, Preclinical/methods
- High-Throughput Screening Assays
- PubMed
- 30836799 Full text @ Expert Opin. Drug Discov.
Citation
Zhao, Y., Zhang, K., Sips, P., MacRae, C.A. (2019) Screening drugs for myocardial disease in vivo with zebrafish: an expert update. Expert opinion on drug discovery. 14(4):343-353.
Abstract
Introduction Our understanding of the complexity of cardiovascular disease pathophysiology remains very incomplete and has hampered cardiovascular drug development over recent decades. The prevalence of cardiovascular diseases and their increasing global burden call for novel strategies to address disease biology and drug discovery. Areas covered: This review describes the recent history of cardiovascular drug discovery using in vivo phenotype-based screening in zebrafish. The rationale for the use of this model is highlighted and the initial efforts in the fields of disease modeling and high-throughput screening are illustrated. Finally, the advantages and limitations of in vivo zebrafish screening are discussed, highlighting newer approaches, such as genome editing technologies, to accelerate our understanding of disease biology and the development of precise disease models. Expert opinion: Full understanding and faithful modeling of specific cardiovascular disease is a rate-limiting step for cardiovascular drug discovery. The resurgence of in vivo phenotype screening together with the advancement of systems biology approaches allows for the identification of lead compounds which show efficacy on integrative disease biology in the absence of validated targets. This strategy bypasses current gaps in knowledge of disease biology and paves the way for successful drug discovery and downstream molecular target identification.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping