PUBLICATION
GSK-3β at the Crossroads in Regulating Protein Synthesis and Lipid Deposition in Zebrafish
- Authors
- Gu, Y., Gao, L., Han, Q., Li, A., Yu, H., Liu, D., Pang, Q.
- ID
- ZDB-PUB-190303-2
- Date
- 2019
- Source
- Cells 8(3): (Journal)
- Registered Authors
- Keywords
- GSK-3β, lipid deposition, muscle, protein synthesis, zebrafish
- MeSH Terms
-
- Zebrafish/metabolism*
- Genetic Vectors/metabolism
- Amino Acids/metabolism
- Models, Biological
- Zebrafish Proteins/metabolism
- Muscles/metabolism
- Animals
- Lipids/chemistry*
- Glycogen Synthase Kinase 3 beta/metabolism*
- Protein Biosynthesis*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- PubMed
- 30823450 Full text @ Cells
Citation
Gu, Y., Gao, L., Han, Q., Li, A., Yu, H., Liu, D., Pang, Q. (2019) GSK-3β at the Crossroads in Regulating Protein Synthesis and Lipid Deposition in Zebrafish. Cells. 8(3).
Abstract
In this study, the mechanism by which GSK-3β regulates protein synthesis and lipid deposition was investigated in zebrafish (Danio rerio). The vector of pEGFP-N1-GSK-3β was constructed and injected into the muscle of zebrafish. It was found that the mRNA and protein expression of tuberous sclerosis complex 2 (TSC2) was significantly increased. However, the mRNA and protein expression of mammalian target of rapamycin (mTOR), p70 ribosomal S6 kinase 1 (S6K1), and 4E-binding protein 1 (4EBP1) was significantly decreased by the pEGFP-N1-GSK-3β vector in the muscle of zebrafish. In addition, the mRNA and protein expression of β-catenin, CCAAT/enhancer binding protein α (C/EBPα), and peroxisome proliferators-activated receptor γ (PPARγ) was significantly decreased, but the mRNA expression of fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), ATP-citrate lyase (ACL), and HMG-CoA reductase (HMGCR) was significantly increased by the pEGFP-N1-GSK-3β vector. The activity of FAS, ACC, ACL, and HMGCR as well as the content of triglyceride (TG), total cholesterol (TC), and nonesterified fatty acids (NEFA) were significantly increased by the pEGFP-N1-GSK-3β vector in the muscle of zebrafish. The content of free amino acids Arg, Lys, His, Phe, Leu, Ile, Val, and Thr was significantly decreased by the pEGFP-N1-GSK-3β vector. The results indicate that GSK-3β may participate in regulating protein synthesis via TSC2/mTOR signaling and regulating lipid deposition via β-catenin in the muscle of zebrafish (Danio rerio).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping