PUBLICATION
Stimulatory effects of NESFATIN-1 on meiotic and developmental competence of porcine oocytes
- Authors
- Cao, Z., Luo, L., Yang, J., Zhang, L., Gao, D., Xu, T., Tong, X., Zhang, D., Wang, Y., Li, Y., Fang, F., Zhang, Y.
- ID
- ZDB-PUB-190227-19
- Date
- 2019
- Source
- Journal of Cellular Physiology 234(10): 17767-17774 (Journal)
- Registered Authors
- Yang, Jie
- Keywords
- NESFATIN-1, granulosa cells, meiotic maturation, oocytes, pig
- MeSH Terms
-
- Animals
- Blastocyst/metabolism
- Blastocyst/physiology
- Cells, Cultured
- Female
- Granulosa Cells/metabolism
- Granulosa Cells/physiology
- Meiosis/physiology*
- Nucleobindins/metabolism*
- Oocytes/metabolism*
- Oocytes/physiology*
- Oogenesis/physiology
- Phylogeny
- Swine
- PubMed
- 30805939 Full text @ J. Cell. Physiol.
Citation
Cao, Z., Luo, L., Yang, J., Zhang, L., Gao, D., Xu, T., Tong, X., Zhang, D., Wang, Y., Li, Y., Fang, F., Zhang, Y. (2019) Stimulatory effects of NESFATIN-1 on meiotic and developmental competence of porcine oocytes. Journal of Cellular Physiology. 234(10):17767-17774.
Abstract
NESFATIN-1 acts as a neuroendocrine hormone to suppress gonadotropin secretion in the female goldfish and to prevent germinal vesicle breakdown of oocytes in the zebrafish. However, the expression and function of NESFATIN-1 in meiotic maturation and development of porcine oocytes remains elusive. Genomic structure of porcine NESFATIN-1 precursor nucleobindin 2 (NUCB2) is first characterized in detail and an evolutionally closer relationship of NESFATIN-1 between pig and rat is shown by phylogenetic analysis of multiple species. Additionally, immunofluorescence analysis revealed that NESFATIN-1 is predominantly expressed and localizes on the membrane of both theca cells and granulosa cells, but not expressed in oocytes. Real-time quantitative polymerase chain reaction showed that the abundance of NESFATIN-1 transcripts in granulosa cells progressively decreases during the developmental transition from small follicles to large follicles. Correspondingly, NESFATIN-1 could significantly enhance both the cleavage and blastocyst rate of parthenogenetically activated oocytes from small follicles (p < 0.05), whereas it did not affect meiotic maturation and development of oocytes from large follicles. Interestingly, we found that NESFATIN-1 significantly improves meiotic maturation of oocytes cultured in chemically defined medium in the absence of pyruvate compared with the control group (p < 0.05), suggesting that the NESFATIN-1 as a substitute for pyruvate exerts beneficial effects on porcine oocyte maturation. In conclusion, these results demonstrate that NESFATIN-1 facilitates both meiotic maturation and development of porcine oocytes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping