ZFIN ID: ZDB-PUB-190210-6
Visualizing the Balbiani Body in Zebrafish Oocytes
Lee, K.L., Marlow, F.L.
Date: 2019
Source: Methods in molecular biology (Clifton, N.J.)   1920: 277-293 (Chapter)
Registered Authors: Marlow, Florence
Keywords: Balbiani body, Buckyball, Histology, Immunohistochemistry, Microscopy, Mitochondria, Oocyte, Primary oocytes, RNA and protein localization
MeSH Terms:
  • Animals
  • Biomarkers
  • Endoplasmic Reticulum/metabolism
  • Female
  • Fluorescent Antibody Technique
  • Immunohistochemistry
  • In Situ Hybridization
  • Mitochondria/metabolism
  • Molecular Imaging/methods*
  • Oocytes/metabolism*
  • Oogenesis*
  • RNA/genetics
  • RNA/metabolism
  • Zebrafish*
PubMed: 30737697 Full text @ Meth. Mol. Biol.
ABSTRACT
Approaches to visualize the Balbiani body of zebrafish primary oocytes using protein, RNA, and mitochondrial markers are described. The method involves isolation, histology, staining, and microscopic examination of early zebrafish oocytes. These techniques can be applied to visualize gene products that are localized to the Balbiani body, and when applied to mutants can be used to decipher molecular and genetic pathways acting in Balbiani body development in early oocytes.
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