|ZFIN ID: ZDB-PUB-190210-6|
Visualizing the Balbiani Body in Zebrafish Oocytes
Lee, K.L., Marlow, F.L.
|Source:||Methods in molecular biology (Clifton, N.J.) 1920: 277-293 (Chapter)|
|Registered Authors:||Marlow, Florence|
|Keywords:||Balbiani body, Buckyball, Histology, Immunohistochemistry, Microscopy, Mitochondria, Oocyte, Primary oocytes, RNA and protein localization|
|PubMed:||30737697 Full text @ Meth. Mol. Biol.|
Lee, K.L., Marlow, F.L. (2019) Visualizing the Balbiani Body in Zebrafish Oocytes. Methods in molecular biology (Clifton, N.J.). 1920:277-293.
ABSTRACTApproaches to visualize the Balbiani body of zebrafish primary oocytes using protein, RNA, and mitochondrial markers are described. The method involves isolation, histology, staining, and microscopic examination of early zebrafish oocytes. These techniques can be applied to visualize gene products that are localized to the Balbiani body, and when applied to mutants can be used to decipher molecular and genetic pathways acting in Balbiani body development in early oocytes.
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