PUBLICATION
Cyclin B3 promotes anaphase I onset in oocyte meiosis
- Authors
- Karasu, M.E., Bouftas, N., Keeney, S., Wassmann, K.
- ID
- ZDB-PUB-190207-2
- Date
- 2019
- Source
- The Journal of cell biology 218(4): 1265-1281 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Anaphase*
- Anaphase-Promoting Complex-Cyclosome/genetics
- Anaphase-Promoting Complex-Cyclosome/metabolism
- Animals
- CDC2 Protein Kinase/genetics
- CDC2 Protein Kinase/metabolism
- Cells, Cultured
- Cyclin B/deficiency
- Cyclin B/genetics
- Cyclin B/metabolism*
- Cyclin B1/genetics
- Cyclin B1/metabolism
- Drosophila melanogaster
- Female
- Fertility*
- Gene Expression Regulation, Developmental
- Infertility, Female/genetics
- Infertility, Female/metabolism*
- Infertility, Female/physiopathology
- Mice, Knockout
- Mutation
- Oocytes/metabolism*
- Securin/genetics
- Securin/metabolism
- Signal Transduction
- Time Factors
- Xenopus laevis
- Zebrafish
- PubMed
- 30723090 Full text @ J. Cell Biol.
Citation
Karasu, M.E., Bouftas, N., Keeney, S., Wassmann, K. (2019) Cyclin B3 promotes anaphase I onset in oocyte meiosis. The Journal of cell biology. 218(4):1265-1281.
Abstract
Meiosis poses unique challenges because two rounds of chromosome segregation must be executed without intervening DNA replication. Mammalian cells express numerous temporally regulated cyclins, but how these proteins collaborate to control meiosis remains poorly understood. Here, we show that female mice genetically ablated for cyclin B3 are viable-indicating that the protein is dispensable for mitotic divisions-but are sterile. Mutant oocytes appear normal until metaphase I but then display a highly penetrant failure to transition to anaphase I. They arrest with hallmarks of defective anaphase-promoting complex/cyclosome (APC/C) activation, including no separase activity, high CDK1 activity, and high cyclin B1 and securin levels. Partial APC/C activation occurs, however, as exogenously expressed APC/C substrates can be degraded. Cyclin B3 forms active kinase complexes with CDK1, and meiotic progression requires cyclin B3-associated kinase activity. Cyclin B3 homologues from frog, zebrafish, and fruit fly rescue meiotic progression in cyclin B3-deficient mouse oocytes, indicating conservation of the biochemical properties and possibly cellular functions of this germline-critical cyclin.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping