PUBLICATION
α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload
- Authors
- Camiolo, G., Tibullo, D., Giallongo, C., Romano, A., Parrinello, N.L., Musumeci, G., Di Rosa, M., Vicario, N., Brundo, M.V., Amenta, F., Ferrante, M., Copat, C., Avola, R., Li Volti, G., Salvaggio, A., Di Raimondo, F., Palumbo, G.A.
- ID
- ZDB-PUB-190203-10
- Date
- 2019
- Source
- International Journal of Molecular Sciences 20(3): (Journal)
- Registered Authors
- Keywords
- alpha lipoic acid, iron, oxidative stress, toxicity, zebrafish
- MeSH Terms
-
- Animals
- Autophagy/drug effects
- Cell Line
- Disease Models, Animal
- Ferric Compounds/adverse effects*
- Glutathione/metabolism
- Heme Oxygenase-1/genetics
- Humans
- Iron Chelating Agents/administration & dosage*
- Iron Chelating Agents/pharmacology
- Iron Overload/chemically induced
- Iron Overload/drug therapy*
- Iron Overload/genetics
- Iron Overload/metabolism
- Oxidative Stress/drug effects
- Quaternary Ammonium Compounds/adverse effects*
- Reactive Oxygen Species/metabolism
- Superoxide Dismutase/genetics
- Thioctic Acid/administration & dosage*
- Thioctic Acid/pharmacology
- Zebrafish
- PubMed
- 30708965 Full text @ Int. J. Mol. Sci.
Citation
Camiolo, G., Tibullo, D., Giallongo, C., Romano, A., Parrinello, N.L., Musumeci, G., Di Rosa, M., Vicario, N., Brundo, M.V., Amenta, F., Ferrante, M., Copat, C., Avola, R., Li Volti, G., Salvaggio, A., Di Raimondo, F., Palumbo, G.A. (2019) α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload. International Journal of Molecular Sciences. 20(3):.
Abstract
Iron toxicity is associated with organ injury and has been reported in various clinical conditions, such as hemochromatosis, thalassemia major, and myelodysplastic syndromes. Therefore, iron chelation therapy represents a pivotal therapy for these patients during their lifetime. The aim of the present study was to assess the iron chelating properties of α-lipoic acid (ALA) and how such an effect impacts on iron overload mediated toxicity. Human mesenchymal stem cells (HS-5) and animals (zebrafish, n = 10 for each group) were treated for 24 h with ferric ammonium citrate (FAC, 120 µg/mL) in the presence or absence of ALA (20 µg/mL). Oxidative stress was evaluated by reduced glutathione content, reactive oxygen species formation, mitochondrial dysfunction, and gene expression of heme oxygenase-1b and mitochondrial superoxide dismutase; organ injury, iron accumulation, and autophagy were measured by microscopical, cytofluorimetric analyses, and inductively coupled plasma‒optical mission Spectrometer (ICP-OES). Our results showed that FAC results in a significant increase of tissue iron accumulation, oxidative stress, and autophagy and such detrimental effects were reversed by ALA treatment. In conclusion, ALA possesses excellent iron chelating properties that may be exploited in a clinical setting for organ preservation, as well as exhibiting a good safety profile and low cost for the national health system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping