PUBLICATION
Negative effects of acute cadmium on stress defense, immunity, and metal homeostasis in liver of zebrafish: The protective role of environmental zinc dpre-exposure
- Authors
- Wang, C.C., Si, L.F., Guo, S.N., Zheng, J.L.
- ID
- ZDB-PUB-190129-12
- Date
- 2019
- Source
- Chemosphere 222: 91-97 (Journal)
- Registered Authors
- Keywords
- Inflammation, Metal exposure, Metal homeostasis, Oxidative stress, Zebrafish
- MeSH Terms
-
- Animals
- Cadmium/pharmacology*
- HSP70 Heat-Shock Proteins/metabolism
- Homeostasis/drug effects*
- Inflammation
- Lipid Peroxidation
- Liver/metabolism*
- Stress, Physiological/drug effects
- Transcription Factors
- Water Pollutants, Chemical/pharmacology
- Zebrafish/metabolism*
- Zinc/pharmacology*
- PubMed
- 30690405 Full text @ Chemosphere
Citation
Wang, C.C., Si, L.F., Guo, S.N., Zheng, J.L. (2019) Negative effects of acute cadmium on stress defense, immunity, and metal homeostasis in liver of zebrafish: The protective role of environmental zinc dpre-exposure. Chemosphere. 222:91-97.
Abstract
In the study, zebrafish were exposed to 0 and 200 μg/L Zn for 8 weeks, and then both groups were transferred to water including 0, 100, and 200 μg/L Cd for 4 days, respectively. Acute Cd exposure caused negative effects on stress defense, immune, and metal transport systems by increasing lipid peroxidation, iNOS activity and mRNA levels of il-6 and inos, and decreasing Cu/Zn-SOD and HSP70 levels, and mRNA levels of sod1, cat, hsp70, p65, mtf-1, znt5, zip7, atp7a, and atp7b. Lipid peroxidation was significantly reduced by Zn pre-exposure under Cd exposure, which may be explained by the enhanced stress defense capacity and the weaken inflammatory response. Firstly, Zn pre-exposure increased MTs and HSP70 levels and CAT activity in Cd-free water, which may facilitate fish quick response to Cd. Secondly, Zn pre-exposure reduced Cd accumulation at 100 and 200 μg/L Cd, down-regulated il-6 and il-1β at 100 μg/L Cd and p65 at 200 μg/L Cd, and increased Cu/Zn-SOD and CAT activities at 200 μg/L Cd. Thirdly, Zn pre-exposure alone up-regulated transcription factors (hsf1, hsf2, and mtf-1, and nrf2) and their target genes (sod1, cat, hsp70, and mt2) under Cd exposure in a dose-dependent manner. It should be noted that Zn pre-exposure down-regulated several metal transport genes dramatically at 0 and 100 μg/L Cd, which may be an important mechanism for reducing Cd import into livers. Overall, long-term and environmental Zn pre-exposure mitigated Cd toxicity by the enhanced stress defense capacity and the down-regulated metal transport and inflammatory responses.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping