PUBLICATION
Fenobucarb induces heart failure and cerebral hemorrhage in zebrafish
- Authors
- Zhu, X.Y., Xia, B., Wu, Y.Y., Yang, H., Li, C.Q., Li, P.
- ID
- ZDB-PUB-190128-7
- Date
- 2019
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 209: 34-41 (Journal)
- Registered Authors
- Keywords
- BPMC, Cerebral hemorrhage, Fenobucarb, Heart failure, Zebrafish
- MeSH Terms
-
- Animals
- Apoptosis/drug effects
- Carbamates/toxicity*
- Cardiac Output/drug effects
- Caspases/metabolism
- Cerebral Hemorrhage/chemically induced*
- Cerebral Hemorrhage/complications*
- Cerebral Hemorrhage/pathology
- Cerebral Hemorrhage/physiopathology
- Dilatation, Pathologic
- Erythrocytes/drug effects
- Erythrocytes/metabolism
- Erythrocytes/pathology
- Gene Expression Regulation/drug effects
- Heart Failure/chemically induced*
- Heart Failure/complications*
- Heart Failure/pathology
- Heart Failure/physiopathology
- Hyperemia/complications
- Reactive Oxygen Species/metabolism
- Regional Blood Flow/drug effects
- Water Pollutants, Chemical/toxicity
- Zebrafish/metabolism*
- PubMed
- 30685545 Full text @ Aquat. Toxicol.
Citation
Zhu, X.Y., Xia, B., Wu, Y.Y., Yang, H., Li, C.Q., Li, P. (2019) Fenobucarb induces heart failure and cerebral hemorrhage in zebrafish. Aquatic toxicology (Amsterdam, Netherlands). 209:34-41.
Abstract
The potential risk and toxic mechanisms of fenobucarb (2-sec-butylphenyl methylcarbamate, BPMC) to animals and humans have not been fully elucidated. In this study, zebrafish embryos were exposed to various concentrations of BPMC from 48 hpf (hour post fertilization, hpf) to 72 hpf. We found that BPMC induced severe heart failure with bradycardia, reduced heart contractions, cardiac output and blood flow dynamics；and myocardial apoptosis. BPMC also induced cerebral hemorrhages and blood erythrocyte reduction in a dose-dependent manner. Also observed were increased ROS production and capase 9 and 3/7 activation. The mRNA levels of the ATPase-related gene (atp2a1l), calcium channel-related gene (cacna1ab), sodium channel-related gene (scn5Lab), potassium channel-related gene (kcnq1), the regulatory gene (tnnc1a) for cardiac troponin C, and several apoptosis-related genes were significantly downregulated in zebrafish following BPMC exposure. These results suggest that exposure to BPMC is a possible risk factor to cardiovascular and cerebrovascular systems in animals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping