PUBLICATION
Anti-inflammatory effect of a novel synthetic compound 1-((4-fluorophenyl)thio)isoquinoline in RAW264.7 macrophages and a zebrafish model
- Authors
- Kim, G.R., Yang, J.Y., Hwang, K.S., Kim, S.S., Chae, J.S., Kan, H., Ahn, J.H., Lee, W.M., Ahn, S.H., Lee, Y.M., Bae, M.A., Shin, D.S.
- ID
- ZDB-PUB-190128-5
- Date
- 2019
- Source
- Fish & shellfish immunology 87: 395-400 (Journal)
- Registered Authors
- Keywords
- Anti-Inflammation, Neutrophil, RAW264.7, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/immunology
- Anti-Inflammatory Agents/pharmacology*
- Lipopolysaccharides/physiology*
- Macrophages/drug effects
- Macrophages/immunology*
- Mice
- Neutrophils/drug effects
- Neutrophils/immunology*
- Quinolines/pharmacology*
- RAW 264.7 Cells
- Zebrafish/immunology*
- PubMed
- 30685466 Full text @ Fish Shellfish Immunol.
Citation
Kim, G.R., Yang, J.Y., Hwang, K.S., Kim, S.S., Chae, J.S., Kan, H., Ahn, J.H., Lee, W.M., Ahn, S.H., Lee, Y.M., Bae, M.A., Shin, D.S. (2019) Anti-inflammatory effect of a novel synthetic compound 1-((4-fluorophenyl)thio)isoquinoline in RAW264.7 macrophages and a zebrafish model. Fish & shellfish immunology. 87:395-400.
Abstract
The compound, 1-((4-fluorophenyl)thio)isoquinoline (FPTQ), is a synthetic isoquinoline derivative. To test the anti-inflammatory effect of FPTQ, we used neutrophil-specific transgenic zebrafish Tg(mpx::EGFP)i114 line and lipopolysaccharide (LPS)-stimulated RAW264.7 cells. We also used two different methods, involving tail transection and LPS stimulation in the zebrafish model. Neutrophils translocation in the zebrafish tail-transected model was inhibited by FPTQ. Neutrophil aggregation was also inhibited by FPTQ in the LPS-stimulated zebrafish model. Decreased mRNA expression of the pro-inflammatory cytokine genes, interleukin-1β (il-1β) and interleukin-6 (il-6), was found in zebrafish larvae injected with FPTQ. Additionally, production of nitric oxide was inhibited by FPTQ in RAW264.7 macrophage cells treated with LPS. Moreover, the mRNA expression of Il-1β and Il-6 suppressed by FPTQ treatment in RAW264.7 macrophage cells, and an enzyme immunoassay showed that FPTQ suppressed the secretion of IL-1β and IL-6 in RAW264.7 cells. These results demonstrate that FPTQ reduced inflammatory responses and, therefore, suggest that it may be effective as an anti-inflammatory agent.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping