ZFIN ID: ZDB-PUB-190125-2
Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology
Chen, X., Liu, M., Lou, H., Lu, Y., Zhou, M.T., Ou, R., Xu, Y., Tang, K.F.
Date: 2019
Source: Genome biology   20: 19 (Journal)
Registered Authors:
Keywords: Knockdown, Protein degradation, TRIM21, Trim-away
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Biotechnology/methods*
  • Embryo, Nonmammalian/metabolism
  • Proteolysis*
  • Ribonucleoproteins/metabolism*
  • Zebrafish
PubMed: 30674345 Full text @ Genome Biol.
Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embryos. Trim-Away depletes proteins faster than morpholinos, which enables analysis of protein function during early embryogenesis. Furthermore, Trim-Away can be applied to evaluate the role of maternally contributed proteins in zebrafish embryos. Our findings indicate that Trim-Away is a powerful tool to perform functional analysis of proteins during zebrafish development.