PUBLICATION
ANALYSIS OF TRANSCRIPTIONAL RESPONSE IN ZEBRAFISH ELEUTHEROEMBRYOS EXPOSED TO CLIMBAZOLE: SIGNALING PATHWAYS AND POTENTIAL BIOMARKERS
- Authors
- Zhang, H., Chen, Z.F., Qi, Z., Yan, S.C., Wei, W.W., Liu, G., Cai, Z.
- ID
- ZDB-PUB-190108-6
- Date
- 2019
- Source
- Environmental toxicology and chemistry 38(4): 794-805 (Journal)
- Registered Authors
- Keywords
- aquatic toxicology, biomarkers, embryonic zebrafish, emerging pollutants, signaling pathways, transcriptional effects
- MeSH Terms
-
- Animals
- Biomarkers/metabolism
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Imidazoles/toxicity*
- Larva/drug effects
- Larva/metabolism
- Sex Differentiation/drug effects
- Sex Differentiation/genetics
- Signal Transduction
- Transcription, Genetic/drug effects*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 30614048 Full text @ Environ. Toxicol. Chem.
Citation
Zhang, H., Chen, Z.F., Qi, Z., Yan, S.C., Wei, W.W., Liu, G., Cai, Z. (2019) ANALYSIS OF TRANSCRIPTIONAL RESPONSE IN ZEBRAFISH ELEUTHEROEMBRYOS EXPOSED TO CLIMBAZOLE: SIGNALING PATHWAYS AND POTENTIAL BIOMARKERS. Environmental toxicology and chemistry. 38(4):794-805.
Abstract
Climbazole is an antifungal active ingredient used in personal care products. After application, this chemical reaches the aquatic environment and may pose a risk to fish. Here, we measured the transcriptional effects of essential genes related to a wide range of signaling pathways on zebrafish eleutheroembryos exposed to climbazole at environmentally relevant and predicted worst-case environmental concentrations, and explored the potential biomarkers via partial least squares discriminant analysis. Transcription analysis covering up to 73 genes revealed significant down-regulation of circadian rhythm- and steroidogenesis-related genes in zebrafish embryos-larvae after exposure to environmentally relevant concentrations of climbazole. Climbazole also modulated the transcripts of genes involved in inflammation, oxidative stress, oocyte maturation and sexual differentiation at predicted worst-case environmental concentrations. Besides, mprα, igf3, nr1d1, nr1d2b, cyp19a1a, vtg1, il-1β, and il-8 were chosen as potential biomarkers in embryonic zebrafish following exposure to climbazole. These findings can help us understand the remarkable transcriptional response to climbazole in the early life stage of zebrafish. Upcoming researches should elucidate whether the transcriptional modulation translates into metabolic phenotypes associated with the corresponding signaling pathways.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping