PUBLICATION
An efficient method to generate xenograft tumor models of acute myeloid leukemia and hepatocellular carcinoma in adult zebrafish
- Authors
- Khan, N., Mahajan, N.K., Sinha, P., Jayandharan, G.R.
- ID
- ZDB-PUB-190108-18
- Date
- 2018
- Source
- Blood cells, molecules & diseases 75: 48-55 (Journal)
- Registered Authors
- Keywords
- Acute myeloid leukemia, Alternative animal model, Busulfan, Hepatocellular carcinoma, Immune suppression, Xenotransplantation, Zebrafish
- MeSH Terms
-
- Animals
- Busulfan/pharmacology
- Carcinoma, Hepatocellular/pathology*
- Cell Compartmentation
- Disease Models, Animal*
- Heterografts*
- Humans
- Leukemia, Myeloid, Acute/pathology*
- Liver Neoplasms, Experimental/pathology*
- Methods
- Zebrafish*
- PubMed
- 30616104 Full text @ Blood Cells Mol. Dis.
Citation
Khan, N., Mahajan, N.K., Sinha, P., Jayandharan, G.R. (2018) An efficient method to generate xenograft tumor models of acute myeloid leukemia and hepatocellular carcinoma in adult zebrafish. Blood cells, molecules & diseases. 75:48-55.
Abstract
Zebrafish is emerging as a promising model for the study of human cancers. Several xenograft models of zebrafish have been developed, particularly in larval stages (<48 h post fertilization) when the immune system of fish is not developed. However, xenografting in adult zebrafish requires laborious and transient methods of immune suppression (γ- irradiation or dexamethasone) that limits engraftment and survival of the tumor or fail to recapitulate specific characteristics of malignancies. Thus, the availability of a simple protocol to successfully engraft adult zebrafish, remains a challenge. The current study addresses this limitation and describes a robust method of xenografting in adult zebrafish. We describe a protocol that involves pre-conditioning of Casper, a pigmentation mutant of zebrafish with busulfan that led to a higher rate of engraftment of hepatocellular carcinoma and acute myeloid leukemia cells. To further ascertain the homing characteristics of the injected cancer cells, we transplanted adult zebrafish by two routes of administration and then studied their compartmentalization. This model presents a valuable alternative to rodents to study the biology of these cancers and also a cost-effective platform for evaluation of potential anti-cancer agents.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping