ZFIN ID: ZDB-PUB-190105-3
Live imaging of angiogenesis during cutaneous wound healing in adult zebrafish
Noishiki, C., Yuge, S., Ando, K., Wakayama, Y., Mochizuki, N., Ogawa, R., Fukuhara, S.
Date: 2019
Source: Angiogenesis   22(2): 341-354 (Journal)
Registered Authors: Fukuhara, Shigetomo, Mochizuki, Naoki
Keywords: Angiogenesis, Cutaneous wound healing, Endothelial cells, Pericytes, Zebrafish
MeSH Terms:
  • Aging/physiology
  • Animals
  • Animals, Genetically Modified
  • Embryo, Nonmammalian
  • Microscopy, Confocal/methods
  • Neovascularization, Physiologic/physiology*
  • Optical Imaging/methods*
  • Skin/injuries
  • Skin/pathology
  • Skin/ultrastructure
  • Skin Physiological Phenomena*
  • Video Recording/methods
  • Wound Healing/physiology*
PubMed: 30607697 Full text @ Angiogenesis
Angiogenesis, the growth of new blood vessels from pre-existing vessels, is critical for cutaneous wound healing. However, it remains elusive how endothelial cells (ECs) and pericytes (PCs) establish new blood vessels during cutaneous angiogenesis. We set up a live-imaging system to analyze cutaneous angiogenesis in adult zebrafish. First, we characterized basic structures of cutaneous vasculature. In normal skin tissues, ECs and PCs remained dormant to maintain quiescent blood vessels, whereas cutaneous injury immediately induced angiogenesis through the vascular endothelial growth factor signaling pathway. Tortuous and disorganized vessel networks formed within a few weeks after the injury and subsequently normalized through vessel regression in a few months. Analyses of the repair process of injured single blood vessels revealed that severed vessels elongated upon injury and anastomosed with each other. Thereafter, repaired vessels and adjacent uninjured vessels became tortuous by increasing the number of ECs. In parallel, PCs divided and migrated to cover the tortuous blood vessels. ECs sprouted from the PC-covered tortuous vessels, suggesting that EC sprouting does not require PC detachment from the vessel wall. Thus, live imaging of cutaneous angiogenesis in adult zebrafish enables us to clarify how ECs and PCs develop new blood vessels during cutaneous angiogenesis.