PUBLICATION
Magnetic nanoparticles: a strategy to target the choroidal layer in the posterior segment of the eye
- Authors
- Giannaccini, M., Pedicini, L., De Matienzo, G., Chiellini, F., Dente, L., Raffa, V.
- ID
- ZDB-PUB-181219-18
- Date
- 2017
- Source
- Scientific Reports 7: 43092 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Choroid/drug effects*
- Drug Delivery Systems*
- Embryo, Nonmammalian
- Magnetite Nanoparticles*
- Retinal Pigment Epithelium/drug effects*
- Vascular Endothelial Growth Factor A/administration & dosage
- Zebrafish*
- PubMed
- 28256525 Full text @ Sci. Rep.
Citation
Giannaccini, M., Pedicini, L., De Matienzo, G., Chiellini, F., Dente, L., Raffa, V. (2017) Magnetic nanoparticles: a strategy to target the choroidal layer in the posterior segment of the eye. Scientific Reports. 7:43092.
Abstract
Despite the higher rate of blindness due to population aging, minimally invasive and selective drug delivery to the eye still remains an open challenge, especially in the posterior segment. The retina, the retinal pigment epithelium (RPE) and the choroid are posterior segment cell layers, which may be affected by several diseases. In particular, damages to the choroid are associated with poor prognosis in the most severe pathologies. A drug delivery approach, able to target the choroid, is still missing. Recently, we demonstrated that intravitreally injected magnetic nanoparticles (MNP) are able to rapidly and persistently localise within the RPE in an autonomous manner. In this work we functionalised the MNP surface with the vascular endothelial growth factor, a bioactive molecule capable of transcytosis from the RPE towards more posterior layers. Such functionalisation successfully addressed the MNPs to the choroid, while MNP functionalised with a control polypeptide (poly-L-lysine) showed the same localisation pattern of the naked MNP particles. These data suggest that the combination of MNP with different bioactive molecules could represent a powerful strategy for cell-specific targeting of the eye posterior segment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping