PUBLICATION
Selection Criteria of Zebrafish Male Donors for Sperm Cryopreservation
- Authors
- Diogo, P., Martins, G., Eufrásio, A., Silva, T., Cabrita, E., Gavaia, P.
- ID
- ZDB-PUB-181215-2
- Date
- 2018
- Source
- Zebrafish 16(2): 189-196 (Journal)
- Registered Authors
- Keywords
- donor age, sperm collection frequency, sperm quality, zebrafish sperm
- MeSH Terms
-
- Age Factors
- Animals
- Cell Membrane/physiology*
- Cryopreservation/methods
- Cryopreservation/veterinary*
- Male
- Specimen Handling*
- Sperm Motility/physiology*
- Spermatozoa/physiology*
- Zebrafish/physiology*
- PubMed
- 30523745 Full text @ Zebrafish
Citation
Diogo, P., Martins, G., Eufrásio, A., Silva, T., Cabrita, E., Gavaia, P. (2018) Selection Criteria of Zebrafish Male Donors for Sperm Cryopreservation. Zebrafish. 16(2):189-196.
Abstract
Selection criteria for sperm cryopreservation are highly relevant in zebrafish since sperm quality is particularly variable in this species. Successful cryopreservation depends on high-quality sperm, which can only be ensured by the selection of breeders. Consequently, male selection and management are a priority to improve cryopreservation, and therefore, this study aimed to characterize optimal age and sperm collection frequency in zebrafish. For this purpose, males from wild type (AB) and from a transgenic line [Tg(runx2:eGFP)] were sampled at 6, 8, 12, and 14 months. For each age, sperm were collected at time 0 followed by samplings at 2, 7, and 14 days of rest. Sperm quality was assessed according to motility and membrane viability parameters. Quality assessment showed that Tg(runx2:eGFP) displayed significantly higher motility than AB and younger males showed higher motility in both lines. Sperm collection frequency affected membrane viability. While AB fish recovered sperm viability after 14 days of rest, Tg(runx2:eGFP) could not recover. Consequently, it may be important to study the sperm quality of each zebrafish line before sperm cryopreservation. Taking into consideration the results achieved in both lines, sperm collection should be performed between 6 and 8 months of age with a minimum collection interval of 14 days.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping