PUBLICATION

A transcriptomics analysis of the Tbx5 paralogues in zebrafish

Authors
Boyle Anderson, E.A.T., Ho, R.K.
ID
ZDB-PUB-181212-15
Date
2018
Source
PLoS One   13: e0208766 (Journal)
Registered Authors
Ho, Robert K.
Keywords
none
Datasets
GEO:GSE115640
MeSH Terms
  • Animals
  • Computational Biology
  • Eye/growth & development
  • Eye/metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Heart/growth & development
  • Myocardium/metabolism
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/metabolism*
  • Transcriptome
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
30532148 Full text @ PLoS One
Abstract
TBX5 is essential for limb and heart development. Mutations in TBX5 are associated with Holt-Oram syndrome in humans. Due to the teleost specific genome duplication, zebrafish have two copies of TBX5: tbx5a and tbx5b. Both of these genes are expressed in regions of the lateral plate mesoderm and retina. In this study, we perform comparative RNA sequencing analysis on zebrafish embryos during the stages of lateral plate mesoderm migration. This work shows that knockdown of the Tbx5 paralogues results in altered gene expression in many tissues outside of the lateral plate mesoderm, especially in the somitic mesoderm and the intermediate mesoderm. Specifically, knockdown of tbx5b results in changes in somite size, in the differentiation of vasculature progenitors and in later patterning of trunk blood vessels.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes