PUBLICATION
RNF135 is a positive regulator of IFN expression and involved in RIG-I signaling pathway by targeting RIG-I
- Authors
- Lai, Y., Liang, M., Hu, ., Zeng, Z., Lin, H., Yi, G., Li, M., Liu, Z.
- ID
- ZDB-PUB-181207-18
- Date
- 2018
- Source
- Fish & shellfish immunology 86: 474-479 (Journal)
- Registered Authors
- Keywords
- IFN, RIG-I, RNF135, Ubiquitination, Zebrafish
- MeSH Terms
-
- Animals
- Gene Expression Profiling/veterinary
- Gene Expression Regulation*
- Interferons/genetics*
- Interferons/metabolism
- Signal Transduction/genetics*
- Ubiquitin-Protein Ligases/genetics*
- Ubiquitin-Protein Ligases/metabolism
- Ubiquitination
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 30508673 Full text @ Fish Shellfish Immunol.
Citation
Lai, Y., Liang, M., Hu, ., Zeng, Z., Lin, H., Yi, G., Li, M., Liu, Z. (2018) RNF135 is a positive regulator of IFN expression and involved in RIG-I signaling pathway by targeting RIG-I. Fish & shellfish immunology. 86:474-479.
Abstract
RIG-I-like receptors (RLRs) play a key role in antiviral and inflammatory responses. Increasing evidence indicates that ubiquitination is crucial for regulation of RIG-I signaling pathway. Several ubiquitin ligases were reported to be involved in RIG-I-mediated signal transduction. In the present study, we demonstrated zebrafish RING finger protein 135 (zbRNF135) was a critical player in the regulation of RIG-I signaling pathway. zbRNF135 mRNA was widely expressed in different tissues of zebrafish. The expression of zbRNF135 was up-regulated post poly(I:C) treatment in vivo and in vitro. Furthermore, the expression profiles of RIG-I signaling pathway related genes (LGP2, MDA5, RIG-I, MAVS, TRAF3, IRF3 and IRF7), together with its downstream molecules (IFN1, ISG15, Mx and PKR), were up-regulated by overexpression of zbRNF135 in ZF4 cells. Luciferase and ubiquitination assays revealed that overexpression of zbRNF135 facilitated zebrafish RIG-I (zbRIG-I)-mediated IFN1 promoter activation by mediating K63-linked ubiquitination of zbRIG-I. The co-immunoprecipitation assay showed that zbRNF135 specifically interacted with zbRIG-I. Our study indicated that zbRNF135 participated in innate immune response through modulating RIG-I signaling pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping