PUBLICATION
Toxicity effects of the organic UV-filter 4-Methylbenzylidene camphor in zebrafish embryos
- Authors
- Quintaneiro, C., Teixeira, B., Benedé, J.L., Chisvert, A., Soares, A.M.V.M., Monteiro, M.S.
- ID
- ZDB-PUB-181127-72
- Date
- 2018
- Source
- Chemosphere 218: 273-281 (Journal)
- Registered Authors
- Soares, Amadeu
- Keywords
- Aromatase, Endocrine disruption, Fish embryo test, Heart rate, Neurotransmission, Oxidative stress
- MeSH Terms
-
- Acetylcholinesterase/genetics
- Animals
- Camphor/analogs & derivatives*
- Camphor/toxicity
- Embryo, Nonmammalian/drug effects*
- Endocrine Disruptors/pharmacology
- Endocrine System/drug effects
- Enzyme Induction/drug effects
- Filtration/instrumentation
- Glutathione Transferase/genetics
- Oxidative Stress/drug effects
- Synaptic Transmission/drug effects
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 30472611 Full text @ Chemosphere
Citation
Quintaneiro, C., Teixeira, B., Benedé, J.L., Chisvert, A., Soares, A.M.V.M., Monteiro, M.S. (2018) Toxicity effects of the organic UV-filter 4-Methylbenzylidene camphor in zebrafish embryos. Chemosphere. 218:273-281.
Abstract
Ultraviolet (UV) filters are widely used in personal care products and due to their lipophilicity these chemicals tend to bioaccumulate in the aquatic biota. 4-Methylbenzylidene camphor (4-MBC) is one of the most used UV-filters, and it is commonly detected in freshwater fish tissues. This substance is suspected to be an endocrine disruptor due to its interaction with Hypothalamus-Pituitary-Gonadal (HPG) and HP-Thyroid (HPT)-axis. The main objective of this study was to evaluate the effects of 4-MBC on apical endpoints, biochemical markers and on genes involved in endocrine pathways in Danio rerio. Zebrafish embryos were exposed to 4-MBC (0.083-0.77 mg/l) from 0 to 96 h post-fertilization (hpf). Hatching, heart rate and malformations were the apical endpoints assessed. Alterations on neurotransmission and oxidative stress were evaluated through acetylcholinesterase (AChE), catalase (CAT) and glutathione S-transferase (GST) enzymatic activities. Endocrine effects were analysed by the expression of genes involved in HPG and HPT-axis of embryos exposed 96 h to the EC10 of 4-MBC (0.19 mg/l). Exposure to 4-MBC induced morphological abnormalities during embryonic development, including notochord curvature, delayed absorption of yolk sac and pericardial oedema. Concentration of 0.77 mg/l 4-MBC decreased embryo heart rate at 48h. At neurotransmission level, an induction of AChE at concentrations above 0.15 mg/l was observed. Malformations and decreased heart rate along with alterations observed at neurotransmission level might have compromised zebrafish larvae equilibrium. Glutathione S-transferase induction above 0.15 mg/l 4-MBC suggests activation of detoxification processes. Furthermore, observed brain aromatase gene down-regulation by 4-MBC suggests impairment of normal functioning of HPG axis in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping