ZFIN ID: ZDB-PUB-181127-69
EVI-1 Modulates Arsenic Trioxide induced Apoptosis through JNK signaling pathway in Leukemia Cells
Lang, W., Zhu, J., Chen, F., Cai, J., Zhong, J.
Date: 2018
Source: Experimental cell research   374(1): 140-151 (Journal)
Registered Authors: Chen, Fangyuan, Zhong, Ji-Hua
Keywords: EVI-1, acute leukemia, apoptosis, arsenic trioxide, c-Jun N-terminal kinase
MeSH Terms:
  • Animals
  • Anthracenes/pharmacology
  • Apoptosis/drug effects*
  • Arsenic Trioxide/pharmacology*
  • Cell Line, Tumor
  • Disease Models, Animal
  • Down-Regulation/drug effects
  • Gene Expression Regulation, Leukemic/drug effects
  • Humans
  • Leukemia/genetics
  • Leukemia/metabolism*
  • Leukemia/pathology*
  • MAP Kinase Signaling System/drug effects*
  • MDS1 and EVI1 Complex Locus Protein/metabolism*
  • Models, Biological
  • Neoplasm Proteins/metabolism
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Zebrafish
PubMed: 30472098 Full text @ Exp. Cell Res.
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ABSTRACT
High expression of the oncogene ecotropic viral integration site-1 (EVI-1) is an independent negative prognostic indicator of survival in leukemia patients. This study aimed to examine the effects of arsenic trioxide (ATO) on EVI-1 in acute myeloid leukemia (AML). Mononuclear cells were isolated from the bone marrow and peripheral blood of AML patients and healthy donors. EVI-1 expression in hematopoietic cells was evaluated by RT-qPCR and Western blot analysis. EVI-1 was highly expressed in both primary AML and leukemia cell lines (THP-1 and K562). ATO down-regulated EVI-1 mRNA in zebrafish in vivo as well as in primary leukemia cells and THP-1 and K562 cells in vitro. Additionally, ATO treatment induced apoptosis, down-regulated both EVI-1 mRNA and oncoprotein expression, increased the expression of pro-apoptosis proteins, and decreased the expression of anti-apoptotic proteins in leukemia cells in vitro. EVI-1 expression in leukemia cells (THP-1 and K562) transduced with EVI-1 siRNA was significantly reduced. Silencing EVI-1 had a significant effect on the activation of the JNK pathway and the induction of leukemia cell apoptosis. ATO may downregulate EVI-1 mRNA and oncoprotein levels and block the inhibitory effects of EVI-1 on the JNK pathway, which activates the JNK apoptotic pathway, thereby leading to the apoptosis of EVI-1 in AML patients.
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