PUBLICATION
Nitric oxide regulates intussusceptive-like angiogenesis in wound repair in chicken embryo and transgenic zebrafish models
- Authors
- Vimalraj, S., Pichu, S., Pankajam, T., Dharanibalan, K., Djonov, V., Chatterjee, S.
- ID
- ZDB-PUB-181116-7
- Date
- 2018
- Source
- Nitric oxide : biology and chemistry 82: 48-58 (Journal)
- Registered Authors
- Keywords
- Chorioallantoic membrane, Intussusceptive angiogenesis, Nitric oxide, Tumor endothelial marker 8, Wound healing
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Chick Embryo
- Disease Models, Animal
- Neovascularization, Pathologic/drug therapy*
- Neovascularization, Pathologic/metabolism
- Neovascularization, Pathologic/pathology
- Nitric Oxide/metabolism
- Nitric Oxide/pharmacology*
- Wound Healing/drug effects*
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish/physiology*
- PubMed
- 30439561 Full text @ Nitric Oxide
Citation
Vimalraj, S., Pichu, S., Pankajam, T., Dharanibalan, K., Djonov, V., Chatterjee, S. (2018) Nitric oxide regulates intussusceptive-like angiogenesis in wound repair in chicken embryo and transgenic zebrafish models. Nitric oxide : biology and chemistry. 82:48-58.
Abstract
Angiogenesis is the formation of new blood vessels that occurs by two distinct processes following sprouting angiogenesis (SA) and intussusceptive angiogenesis (IA). Nitric oxide (NO) is known for its pro-angiogenic functions. However, no clear mechanisms are delineated on its role in promoting angiogenesis in reparative wound healing. We propose that NO regulates SA to IA transition and vice versa in wound milieu. We have used three models which include a new chick embryo extra-vasculature (CEV) burn wound model, adult Tie2-GFP transgenic Zebrafish caudal fin regeneration model and Zebrafish skin wound model to study the mechanisms underlying behind the role of NO in wound healing. Wounds created in CEV were treated with NO donor (Spermine NONOate (SPNO)), NOS inhibitor (L-nitro-l-arginine-methyl ester (l-NAME)), NaNO2, NaNO3, and beetroot juice, a nitrite-rich juice respectively and the pattern of wound healing was assessed. Morphological and histological techniques tracked the wound healing at the cellular level, and the molecular changes were investigated by using real-time RT-PCR gene expression analysis. The result concludes that NO donor promotes wound healing by activating SA at an early phase of healing while NOS inhibitor induces wound healing via IA. At the later phase of wound healing NO donor followed IA while NOS inhibitor failed to promote wound repair. The current work underpinned a differential regulation of NO on angiogenesis in wound milieu and this study would provide new insights in designing therapeutics for promoting wound repair.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping