PUBLICATION
Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns
- Authors
- Yamakawa, N., Vanbeselaere, J., Chang, L.Y., Yu, S.Y., Ducrocq, L., Harduin-Lepers, A., Kurata, J., Aoki-Kinoshita, K.F., Sato, C., Khoo, K.H., Kitajima, K., Guerardel, Y.
- ID
- ZDB-PUB-181109-3
- Date
- 2018
- Source
- Nature communications 9: 4647 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Aging/metabolism*
- Animals
- Brain/metabolism
- Gene Expression Regulation
- Glycomics/methods*
- Glycosphingolipids/isolation & purification
- Glycosylation
- Intestines
- N-Acetylneuraminic Acid/metabolism*
- Organ Specificity*
- Polysaccharides/isolation & purification
- Systems Biology/methods*
- Zebrafish/metabolism*
- PubMed
- 30405127 Full text @ Nat. Commun.
Citation
Yamakawa, N., Vanbeselaere, J., Chang, L.Y., Yu, S.Y., Ducrocq, L., Harduin-Lepers, A., Kurata, J., Aoki-Kinoshita, K.F., Sato, C., Khoo, K.H., Kitajima, K., Guerardel, Y. (2018) Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns. Nature communications. 9:4647.
Abstract
The emergence of zebrafish Danio rerio as a versatile model organism provides the unique opportunity to monitor the functions of glycosylation throughout vertebrate embryogenesis, providing insights into human diseases caused by glycosylation defects. Using a combination of chemical modifications, enzymatic digestion and mass spectrometry analyses, we establish here the precise glycomic profiles of eight individual zebrafish organs and demonstrate that the protein glycosylation and glycosphingolipid expression patterns exhibits exquisite specificity. Concomitant expression screening of a wide array of enzymes involved in the synthesis and transfer of sialic acids shows that the presence of organ-specific sialylation motifs correlates with the localized activity of the corresponding glycan biosynthesis pathways. These findings provide a basis for the rational design of zebrafish lines expressing desired glycosylation profiles.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping