ZFIN ID: ZDB-PUB-181106-16
Guidelines and best practices in successfully using Zebrabow for lineage tracing multiple cells within tissues
Nguyen, P.D., Currie, P.D.
Date: 2018
Source: Methods (San Diego, Calif.)   150: 63-67 (Other)
Registered Authors: Currie, Peter D., Nguyen, Phong D.
Keywords: Brainbow, Imaging, Lineage tracing, Zebrabow, Zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Lineage/genetics
  • Cell Tracking/methods
  • Cell Tracking/standards*
  • Embryo, Nonmammalian
  • Genes, Reporter/genetics
  • Guidelines as Topic
  • Integrases/genetics
  • Luminescent Proteins/chemistry
  • Luminescent Proteins/genetics
  • Models, Animal
  • Staining and Labeling/methods
  • Staining and Labeling/standards*
  • Zebrafish*
PubMed: 30392565 Full text @ Methods
ABSTRACT
Labelling cells and following their progeny, also known as lineage tracing, has provided important insights into the cellular origins of tissues. Traditional lineage tracing experiments have been limited to following single or small groups of cells with classic techniques such as dye injections and Cre/LoxP labelling of cells of interest. Brainbow is a fluorescent dependent, lineage tracing technique that allows a broader visualization and analysis of multiple cells within a tissue, initially deployed to examine lineages within neural tissues. This technique has now been adapted to zebrafish (Zebrabow) and takes advantages of the imaging capabilities that this system provides over other animal models. In this paper we shall describe how Zebrabow is performed as well as some guides on some of the common pitfalls encountered when using this labelling strategy.
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