ZFIN ID: ZDB-PUB-181027-10
An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae
Bresciani, E., Broadbridge, E., Liu, P.P.
Date: 2018
Source: MethodsX   5: 1287-1290 (Other)
Registered Authors: Bresciani, Erica, Liu, Pu Paul
Keywords: Dissociation, Larvae dissociation to single cell suspension, Single cell suspension, Zebrafish, Zebrafish embryo
MeSH Terms: none
PubMed: 30364607 Full text @ MethodsX
Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to understand gene function not only during embryonic stages but also in larval stages. Therefore, there is an increasing need to optimize methods for embryo and larvae dissociation that allow the generation of single cell suspension for fluorescence-activated cell sorting (FACS), RNA extraction and single cell RNA-sequencing.