PUBLICATION

Generation and characterization of a zebrafish muscle specific inducible Cre line

Authors
Mukherjee, K., Liao, E.C.
ID
ZDB-PUB-181026-7
Date
2018
Source
Transgenic Research   27(6): 559-569 (Journal)
Registered Authors
Liao, Eric
Keywords
Cre/loxP, Inducible, Muscles, Tissue-specific, Transgenic, Zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism*
  • Gene Expression Regulation, Developmental
  • Integrases/genetics
  • Integrases/metabolism*
  • Muscles/cytology
  • Muscles/metabolism*
  • Transgenes*
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
PubMed
30353407 Full text @ Transgenic. Res.
Abstract
Zebrafish transgenic lines provide valuable insights into gene functions, cell lineages and cell behaviors during development. Spatiotemporal control over transgene expression is a critical need in many experimental approaches, with applications in loss- and gain-of-function expression, ectopic expression and lineage tracing experiments. The Cre/loxP recombination system is a powerful tool to provide this control and the demand for validated Cre and loxP zebrafish transgenics is high. One of the major challenges to widespread application of Cre/loxP technology in zebrafish is comparatively small numbers of established tissue-specific Cre or CreERT2 lines. We used Tol2-mediated transgenesis to generate Tg(CrymCherry;-1.9mylz2:CreERT2) which provides an inducible CreERT2 source driven by muscle-specific mylz2 promoter. The transgenic specifically labels the trunk and tail skeletal muscles. We assessed the temporal responsiveness of the transgenic by screening with a validated loxP reporter transgenic ubi:Switch. Further, we evaluated the recombination efficiency in the transgenic with varying concentrations of 4-OHT, for different induction time periods and at different stages of embryogenesis and observed that higher recombination efficiency is achieved when embryos are induced with 10 μM 4-OHT from 10-somites or 24 hpf till 48 or 72 hpf. The transgenic is an addition to currently available zebrafish transgenesis toolbox and a significant tool to advance muscle biology studies in zebrafish.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes