ZFIN ID: ZDB-PUB-181023-8
Sequential Specification of Oligodendrocyte Lineage Cells by Distinct Levels of Hedgehog and Notch Signaling
Ravanelli, A.M., Kearns, C.A., Powers, R.K., Wang, Y., Hines, J.H., Donaldson, M.J., Appel, B.
Date: 2018
Source: Developmental Biology   444(2): 93-106 (Journal)
Registered Authors: Appel, Bruce, Hines, Jacob H., Kearns, Christina, Ravanelli, Andrew M.
Keywords: none
MeSH Terms:
  • Animals
  • Brain/metabolism
  • Cell Differentiation/physiology
  • Cell Lineage
  • Cell Proliferation
  • Central Nervous System/metabolism
  • Hedgehog Proteins/metabolism*
  • Neuroglia/metabolism
  • Oligodendrocyte Precursor Cells/metabolism*
  • Oligodendrocyte Precursor Cells/physiology
  • Oligodendroglia/physiology
  • Receptors, Notch/metabolism*
  • Signal Transduction/physiology
  • Spinal Cord/metabolism
  • Stem Cells/metabolism
  • Zebrafish/embryology
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 30347186 Full text @ Dev. Biol.
ABSTRACT
During development of the central nervous system oligodendrocyte precursor cells (OPCs) give rise to both myelinating oligodendrocytes and NG2 glia, which are the most proliferative cells in the adult mammalian brain. NG2 glia retain characteristics of OPCs, and some NG2 glia produce oligodendrocytes, but many others persist throughout adulthood. Why some OPCs differentiate as oligodendrocytes during development whereas others persist as OPCs and acquire characteristics of NG2 glia is not known. Using zebrafish spinal cord as a model, we found that OPCs that differentiate rapidly as oligodendrocytes and others that remain as OPCs arise in sequential waves from distinct neural progenitors. Additionally, oligodendrocyte and persistent OPC fates are specified during a defined critical period by small differences in Shh signaling and Notch activity, which modulates Shh signaling response. Thus, our data indicate that OPCs fated to produce oligodendrocytes or remain as OPCs during development are specified as distinct cell types, raising the possibility that the myelinating potential of OPCs is set by graded Shh signaling activity.
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