ZFIN ID: ZDB-PUB-181020-6
Functional characterization of Cynoglossus semilaevis R-spondin2 and its role in muscle development during embryogenesis
Wang, B., Yang, F., Li, R., Li, X., Wu, X., Sun, Z., Zhai, J., He, Y., Qi, J.
Date: 2018
Source: Genes & genetic systems   93(5): 181-190 (Journal)
Registered Authors: He, Yan, Qi, Jie, Wang, Bo
Keywords: Cynoglossus semilaevis, R-spondin2, Wnt signaling pathway, muscle development
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Embryonic Development
  • Female
  • Flatfishes/embryology
  • Flatfishes/genetics
  • Flatfishes/physiology
  • Intercellular Signaling Peptides and Proteins/genetics*
  • Intercellular Signaling Peptides and Proteins/physiology*
  • Male
  • Muscle Development/genetics*
  • Muscle Development/physiology
  • Phylogeny
  • RNA, Messenger/genetics
  • Xenopus Proteins/genetics
  • Zebrafish
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/physiology*
PubMed: 30333384 Full text @ Genes Genet. Sys.
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ABSTRACT
R-spondin2 (Rspo2) is a member of the R-spondin family, which plays important roles in cell proliferation, cell fate determination and organogenesis. Rspo2 exhibits important functions during embryonic development and muscle maintenance in adult human, mouse and Xenopus. In the present study, the tongue sole Cynoglossus semilaevis Rspo2 (CsRspo2) gene was isolated and characterized, and its role in muscle development during embryogenesis was studied. Our results showed that CsRspo2 expression was abundant during gastrulation and significantly high during somite formation, but then decreased markedly after hatching. CsRspo2 expression was high in brain and gill, moderate in heart, ovary and testis, and almost undetectable in muscle and other tissues. Moreover, the potential involvement of Rspo2 in muscle development was investigated. We found that overexpression of CsRspo2 mRNA in zebrafish embryos resulted in slow development and abnormal muscle formation at the embryonic stage. Our work provides a fundamental understanding of the structure and potential functions of CsRspo2 during muscle development.
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