PUBLICATION
Characterization of Zebrafish Facial Lymphatics
- Authors
- Eng, T.C.Y., Astin, J.W.
- ID
- ZDB-PUB-180923-8
- Date
- 2018
- Source
- Methods in molecular biology (Clifton, N.J.) 1846: 71-83 (Chapter)
- Registered Authors
- Astin, Jonathan
- Keywords
- Confocal, Facial, Head, Live imaging, Lymphatic, Zebrafish
- MeSH Terms
-
- Animals
- Gene Expression
- Genes, Reporter
- Larva
- Lymphangiogenesis*
- Lymphatic Vessels/cytology
- Lymphatic Vessels/embryology
- Lymphatic Vessels/metabolism*
- Molecular Imaging/methods
- Time Factors
- Time-Lapse Imaging/methods
- Zebrafish*/embryology
- PubMed
- 30242753 Full text @ Meth. Mol. Biol.
Citation
Eng, T.C.Y., Astin, J.W. (2018) Characterization of Zebrafish Facial Lymphatics. Methods in molecular biology (Clifton, N.J.). 1846:71-83.
Abstract
Zebrafish are well-established as a model of vascular development. The genetic tractability, external development, permeability to small molecules and optical transparency of zebrafish larvae are all attributes that make this model attractive to the vascular biologist. There are an increasing number of lymphatic reporter lines that enable the visualization of zebrafish lymphatic vessel growth in vivo; these tools, coupled with either forward or reverse genetics, have provided new insights into the process of lymphatic specification and development. Zebrafish larvae have three main lymphatic networks: the trunk lymphatics, the intestinal lymphatics, and the facial lymphatics and it is therefore possible to use zebrafish to determine network-specific roles for molecules implicated in lymphatic development. This chapter provides protocols for visualization and analysis of facial lymphatic development in the zebrafish and may be applied in developmental or drug discovery studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping