PUBLICATION
Uptake, depuration, dose estimation and effects in zebrafish exposed to Am-241 via dietary route
- Authors
- Simon, O., Barjhoux, I., Camilleri, V., Gagnaire, B., Cavalié, I., Orjollet, D., Darriau, F., Pereira, S., Beaugelin-Seillers, K., Adam-Guillermin, C.
- ID
- ZDB-PUB-180913-4
- Date
- 2018
- Source
- Journal of Environmental Radioactivity 193-194: 68-74 (Journal)
- Registered Authors
- Keywords
- Americium, Effects, Genes expression, Genotoxicity, Zebrafish
- MeSH Terms
-
- Americium/metabolism*
- Animals
- Dietary Exposure*
- Radiation Dosage*
- Water Pollutants, Radioactive/metabolism*
- Zebrafish/physiology*
- PubMed
- 30199762 Full text @ J. Environ. Radioact.
Citation
Simon, O., Barjhoux, I., Camilleri, V., Gagnaire, B., Cavalié, I., Orjollet, D., Darriau, F., Pereira, S., Beaugelin-Seillers, K., Adam-Guillermin, C. (2018) Uptake, depuration, dose estimation and effects in zebrafish exposed to Am-241 via dietary route. Journal of Environmental Radioactivity. 193-194:68-74.
Abstract
Zebrafish were chronically exposed to Am-241, an alpha-emitting radionuclide via daily consumption of an enriched artificial diet. Am-241 uptake was quantified in Danio rerio after 5 and 21 days of exposure via daily Am-spiked food ingestion and after 21 days of exposure followed by 5 days of depuration. Americium accumulates mostly in digestive tract, muscle, rest of the body but the accumulation levels and trophic transfer rate (0.033-0.013%) were low. Corresponding cumulative doses were calculated for the whole body (9 mGy) and for the digestive tract (42 mGy) with internal alpha radiation contributing to more than 99% of the total dose. Genotoxic effects (gamma-H2AX assay) and differential gene expressions of main biological functions were examined. Although fish were exposed to a low dose rate of 13 μGy h-1, DNA integrity and gene expression linked to oxidative stress, hormonal signaling and spermatogenesis were altered after 21 days of Am-241 exposure. These results underline the higher toxicity of alpha emitter Am-241, as compared to other studies on gamma radiation exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping