Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis

He, W.X., Wu, M., Liu, Z., Li, Z., Wang, Y., Zhou, J., Yu, P., Zhang, X.J., Zhou, L., Gui, J.F.
RNA (New York, N.Y.)   24(12): 1738-1748 (Journal)
Registered Authors
Gui, Jian-Fang, Li, Zhi, Wang, Yang, Zhang, Xiao-juan, Zhou, Li
maternal effect, oogenesis, replication-dependent histones, ribosomal proteins, slbp2
MeSH Terms
  • Animals
  • Cell Nucleus/genetics
  • DNA Replication/genetics
  • Embryonic Development/genetics*
  • Gene Expression Regulation, Developmental
  • Histones/genetics*
  • Oocytes/growth & development
  • Oogenesis/genetics*
  • RNA-Binding Proteins/genetics*
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zygote/growth & development
  • mRNA Cleavage and Polyadenylation Factors/genetics
30185624 Full text @ RNA
Stem-loop binding protein (SLBP) has been revealed to bind to the stem-loop of replication-dependent histone mRNAs and to be required for replication-dependent histone mRNA metabolism in mammals. Zebrafish possesses two slbps and slbp1 is required for retinal neurogenesis. However, the detailed expression and function of slbp2 in zebrafish is still unknown. In this study, we first identified zebrafish slbp2 as an oocyte-specific maternal factor and then generated maternal-zygotic slbp2 F3 homozygous mutant (MZslbp2Δ4-/-) by CRISPR/Cas9. The depletion of maternal Slbp2 caused the disruption of the early nuclear cleavage, which resulted in development arrest at MBT stage. The developmental defects could be rescued in slbp2 transgenic MZslbp2Δ4-/- embryos. Through comparative proteome and transcriptome profiling between WT and MZslbp2Δ4-/- embryos, many differentially expressed proteins and genes were identified, and most of them were histones and ribosomal proteins. Compared to WT embryos, four replication-dependent histones, including H2a, H2b, H3 and H4, were all dramatically reduced their expression, while histone variant h2afx significantly increased in MZslbp2Δ4-/- embryos at 256-cell stage and high stage. Zebrafish Slbp2 can bind histone mRNAs stem-loop in vitro and the defects of MZslbp2Δ4-/- embryos can be partially rescued by overexpression of H2b. Additionally, many ribosomal proteins, such as three members of P proteins, were confirmed to raise their expression in MZslbp2Δ4-/- embryos, indicating the association between protein synthesis disturbances and abnormal nuclear cleavage in MZslbp2Δ4-/- embryos. The current study revealed that maternal Slbp2 plays pivotal role in early nuclear cleavage and replication-dependent histone expression in zebrafish embryogenesis.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes