PUBLICATION
Staufen1, Kinesin1 and microtubule function in cyclin B1 mRNA transport to the animal polar cytoplasm of zebrafish oocytes
- Authors
- Takahashi, K., Ishii, K., Yamashita, M.
- ID
- ZDB-PUB-180816-1
- Date
- 2018
- Source
- Biochemical and Biophysical Research Communications 503(4): 2778-2783 (Journal)
- Registered Authors
- Keywords
- Kinesin1, Microtubule, Staufen1, Zebrafish oocyte, mRNA transport
- MeSH Terms
-
- Animals
- Antibodies, Neutralizing/pharmacology
- Biological Transport
- Cell Polarity/genetics
- Cyclin B1/antagonists & inhibitors
- Cyclin B1/genetics*
- Cyclin B1/metabolism
- Female
- Gene Expression Regulation, Developmental
- Kinesins/genetics*
- Kinesins/metabolism
- Microtubules/metabolism
- Microtubules/ultrastructure
- Oocytes/growth & development
- Oocytes/metabolism*
- Oocytes/ultrastructure
- Oogenesis/genetics
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- RNA-Binding Proteins/genetics*
- RNA-Binding Proteins/metabolism
- Signal Transduction
- Time-Lapse Imaging
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/metabolism
- Xenopus Proteins/genetics
- Xenopus Proteins/metabolism
- Xenopus laevis/genetics*
- Xenopus laevis/growth & development
- Xenopus laevis/metabolism
- Zebrafish/genetics*
- Zebrafish/growth & development
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 30103945 Full text @ Biochem. Biophys. Res. Commun.
Citation
Takahashi, K., Ishii, K., Yamashita, M. (2018) Staufen1, Kinesin1 and microtubule function in cyclin B1 mRNA transport to the animal polar cytoplasm of zebrafish oocytes. Biochemical and Biophysical Research Communications. 503(4):2778-2783.
Abstract
In zebrafish oocytes, cyclin B1 mRNAs are transported to the animal polar cytoplasm. To elucidate the molecular basis of cyclin B1 mRNA transport, we analyzed zebrafish Staufen1, a protein known to play a central role in mRNA transport to the vegetal pole of Xenopus oocytes. Zebrafish Staufen1 interacts with cyclin B1 mRNA throughout oocyte growth. Both cyclin B1 mRNA and Staufen1 are evenly distributed in the cytoplasm of young oocytes but are co-localized to the animal polar cytoplasm in later stages. Real-time imaging showed that the plus ends of oocyte microtubules are free in the cytoplasm in early stages but anchored to the animal polar cytoplasm in later stages. Transport of cyclin B1 reporter mRNA to the animal polar cytoplasm was inhibited by disruption of microtubules and injection of antibodies against Staufen1 or Kinesin1, a plus-end-directed microtubule motor that interacts with Staufen1, indicating that the transport depends on movement along microtubules toward the plus ends. Reporter mRNAs with an element required for the vegetal localization of vg1 mRNA in Xenopus oocytes were localized to the animal polar cytoplasm in zebrafish oocytes, indicating that the element is functional for animal polar localization in zebrafish oocytes. Our findings suggest that cyclin B1 mRNA-Staufen1 protein complexes are transported toward the animal pole of zebrafish oocytes by the plus-end-directed motor protein Kinesin1 along microtubules and that a common mRNA transport machinery functions in zebrafish and Xenopus oocytes, although its transport direction is opposite due to different organizations of microtubules.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping