PUBLICATION
Neuroanatomical localization of galanin in zebrafish telencephalon and anticonvulsant effect of galanin overexpression
- Authors
- Podlasz, P., Jakimiuk, A., Kasica-Jarosz, N., Czaja, K., Wasowicz, K.
- ID
- ZDB-PUB-180811-6
- Date
- 2018
- Source
- ACS Chemical Neuroscience 9(12): 3049-3059 (Journal)
- Registered Authors
- Jakimiuk, Anna, Podlasz, Piotr, Wasowicz, Krzysztof
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Convulsants/toxicity
- Galanin/genetics*
- Galanin/metabolism
- HSP70 Heat-Shock Proteins/genetics
- HSP70 Heat-Shock Proteins/metabolism
- Heat-Shock Response
- Locomotion
- Pentylenetetrazole/toxicity
- Proto-Oncogene Proteins c-fos/drug effects
- Proto-Oncogene Proteins c-fos/metabolism
- Seizures/chemically induced
- Seizures/genetics*
- Seizures/metabolism
- Telencephalon/metabolism*
- Zebrafish
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 30095254 Full text @ ACS Chem. Neurosci.
Citation
Podlasz, P., Jakimiuk, A., Kasica-Jarosz, N., Czaja, K., Wasowicz, K. (2018) Neuroanatomical localization of galanin in zebrafish telencephalon and anticonvulsant effect of galanin overexpression. ACS Chemical Neuroscience. 9(12):3049-3059.
Abstract
Galanin is a neuropeptide widely expressed in the nervous system, but it is also present in nonneuronal locations. In the brain galanin may function as an inhibitory neurotransmitter. Several studies have showed that galanin is involved in seizure regulation and can modulate epileptic activity in the brain. The overall goal of the study was to establish a zebrafish as a model to study the antiepileptic effect of galanin. The goal of this study was achieved by: 1) determining neuroanatomical localization of galanin in zebrafish lateral pallium, which is considered to be the zebrafish homologue of the mammalian hippocampus, the brain region essential for initiation of seizures; and 2) testing the anticonvulsant effect of galanin overexpression. Whole mount immunofluorescence staining and pentylenotetrazole(PTZ)-seizure model in larval zebrafish using automated analysis of motor function and qPCR were used in the study. Immunohistochemical staining of zebrafish larvae revealed numerous galanin-IR fibers innervating the subpallium, but only scarce fibers reaching the dorsal parts of telencephalon, including lateral pallium. In 3-month old zebrafish galanin-IR innervation of the telencephalon was similar, however much more galanin-IR fibers reached the dorsal telencephalon, but in the lateral pallium only scarce galanin-IR fibers were visible. qRT-PCR revealed, as expected, a strong increase in the expression of galanin in Tg(hsp70l:galn) line after heat shock, however also without heat shock the galanin expression was several-fold higher than in the control animals. Galanin overexpression resulted in downregulation of c-fos after PTZ treatment. Behavioral analysis showed that galanin overexpression inhibited locomotor activity in PTZ-treated and control larvae. The obtained results show that galanin overexpression reduced the incidence of seizure-like behavior episodes and their intensity but had no significant effect on their duration. The findings indicate that in addition to the antiepileptic action, galanin modulates the arousal behavior and demonstrates a sedative effect. The current study showed that galanin overexpression correlated with a potent anticonvulsant effect in the zebrafish PTZ seizure-model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping