ZFIN ID: ZDB-PUB-180809-7
Pitpnc1a Regulates Zebrafish Sleep and Wake Behavior through Modulation of Insulin-like Growth Factor Signaling
Ashlin, T.G., Blunsom, N.J., Ghosh, M., Cockcroft, S., Rihel, J.
Date: 2018
Source: Cell Reports   24: 1389-1396 (Journal)
Registered Authors: Ghosh, Marcus, Rihel, Jason
Keywords: IGF, behavior, lipid transporter, sleep, zebrafish
MeSH Terms:
  • Animals
  • Insulin-Like Growth Factor I/metabolism*
  • Membrane Transport Proteins/pharmacology
  • Membrane Transport Proteins/therapeutic use*
  • Signal Transduction
  • Wakefulness/physiology*
  • Zebrafish
PubMed: 30089250 Full text @ Cell Rep.
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ABSTRACT
The lipid transporters of the phosphatidylinositol transfer protein (PITP) family dictate phosphoinositide compartmentalization, and specific phosphoinositides play crucial roles in signaling cascades, membrane traffic, ion channel regulation, and actin dynamics. Although PITPs are enriched in the brain, their physiological functions in neuronal signaling pathways in vivo remain ill defined. We describe a CRISPR/Cas9-generated zebrafish mutant in a brain-specific, conserved class II PITP member, pitpnc1a. Zebrafish pitpnc1a mutants are healthy but display widespread aberrant neuronal activity and increased wakefulness across the day-night cycle. The loss of Pitpnc1a increases insulin-like growth factor (IGF) signaling in the brain, and inhibition of IGF pathways is sufficient to rescue both neuronal and behavioral hyperactivity in pitpnc1a mutants. We propose that Pitpnc1a-expressing neurons alter behavior via modification of neuro-modulatory IGF that acts on downstream wake-promoting circuits.
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