PUBLICATION
MTG1 couples mitoribosome large subunit assembly with intersubunit bridge formation
- Authors
- Kim, H.J., Barrientos, A.
- ID
- ZDB-PUB-180808-14
- Date
- 2018
- Source
- Nucleic acids research 46(16): 8435-8453 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Cardiomyopathies/genetics
- Cardiomyopathies/pathology
- GTP Phosphohydrolases/biosynthesis
- GTP Phosphohydrolases/genetics*
- Gene Silencing
- HEK293 Cells
- Humans
- Metalloproteins/genetics*
- Mitochondria/genetics*
- Mitochondria/metabolism
- Mitochondrial Ribosomes/metabolism*
- Myocytes, Cardiac/metabolism
- Myocytes, Cardiac/pathology
- Nuclear Proteins/genetics*
- Oxidative Phosphorylation
- RNA, Ribosomal, 16S/genetics
- RNA-Binding Proteins/genetics*
- Ribosomal Proteins/genetics*
- Ribosomes/genetics*
- Zebrafish/genetics
- Zebrafish/growth & development
- PubMed
- 30085276 Full text @ Nucleic Acids Res.
Citation
Kim, H.J., Barrientos, A. (2018) MTG1 couples mitoribosome large subunit assembly with intersubunit bridge formation. Nucleic acids research. 46(16):8435-8453.
Abstract
Mammalian mitochondrial ribosomes (mitoribosomes) synthesize 13 proteins, essential components of the oxidative phosphorylation system. They are linked to mitochondrial disorders, often involving cardiomyopathy. Mitoribosome biogenesis is assisted by multiple cofactors whose specific functions remain largely uncharacterized. Here, we examined the role of human MTG1, a conserved ribosome assembly guanosine triphosphatase. MTG1-silencing in human cardiomyocytes and developing zebrafish revealed early cardiovascular lesions. A combination of gene-editing and biochemical approaches using HEK293T cells demonstrated that MTG1 binds to the large subunit (mtLSU) 16S ribosomal RNA to facilitate incorporation of late-assembly proteins. Furthermore, MTG1 interacts with mtLSU uL19 protein and mtSSU mS27, a putative guanosine triphosphate-exchange factor (GEF), to enable MTG1 release and the formation of the mB6 intersubunit bridge. In this way, MTG1 establishes a quality control checkpoint in mitoribosome assembly. In conclusion, MTG1 controls mitochondrial translation by coupling mtLSU assembly with intersubunit bridge formation using the intrinsic GEF activity acquired by the mtSSU through mS27, a unique occurrence in translational systems.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping